CAPG Human

Capping Protein Gelsolin-Like Human Recombinant
Cat. No.
BT4193
Source
Escherichia Coli.
Synonyms
AFCP, CAPG, Macrophage-capping protein, Actin regulatory protein CAP-G, MCP.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CAPG Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 348 amino acids (1-348 a.a.) and having a molecular mass of 38.5 kDa. The CAPG protein is purified by standard chromatography techniques.

Product Specs

Introduction
CAPG, a member of the gelsolin/villin family, regulates actin dynamics. This protein reversibly binds to the barbed ends of filamentous actin (F-actin) in a calcium (Ca2+) and phosphoinositide-dependent manner. Unlike severing proteins, CAPG stabilizes existing actin filaments by preventing further polymerization or depolymerization at the barbed end. This capping activity is crucial for controlling actin-based cellular processes, particularly cell motility in non-muscle cells. Beyond its role in actin regulation, CAPG participates in macrophage function and potentially influences cytoplasmic and nuclear structures through interactions with actin. Additionally, CAPG exhibits DNA-binding properties and lacks a nuclear export signal commonly found in related proteins. Notably, CAPG functions as a tumor suppressor, and its dysregulation is implicated in the progression of certain cancers, including oral carcinogenesis.
Description
Recombinant human CAPG, expressed in E. coli, is a non-glycosylated polypeptide consisting of 348 amino acids (1-348 a.a.). With a molecular weight of 38.5 kDa, this protein is purified to a high degree using standardized chromatography techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
This protein solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris (pH 8), 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of this protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
AFCP, CAPG, Macrophage-capping protein, Actin regulatory protein CAP-G, MCP.
Source
Escherichia Coli.
Amino Acid Sequence
MYTAIPQSGS PFPGSVQDPG LHVWRVEKLK PVPVAQENQG VFFSGDSYLV LHNGPEEVSH LHLWIGQQSS RDEQGACAVL AVHLNTLLGE RPVQHREVQG NESDLFMSYF PRGLKYQEGG VESAFHKTST GAPAAIKKLY QVKGKKNIRA TERALNWDSF NTGDCFILDL GQNIFAWCGG KSNILERNKA RDLALAIRDS ERQGKAQVEI VTDGEEPAEM IQVLGPKPAL KEGNPEEDLT ADKANAQAAA LYKVSDATGQ MNLTKVADSS PFALELLISD DCFVLDNGLC GKIYIWKGRK ANEKERQAAL QVAEGFISRM QYAPNTQVEI LPQGRESPIF KQFFKDWK.

Product Science Overview

Introduction

Capping Protein Gelsolin-Like, also known as CapG, is a member of the gelsolin family of actin-modulating proteins. These proteins play a crucial role in the regulation of the actin cytoskeleton, which is essential for various cellular processes such as cell signaling, membrane ruffling, phagocytosis, and motility .

Structure and Function

CapG is a calcium-sensitive protein that binds to the barbed ends of actin filaments, thereby capping them and preventing further polymerization. Unlike some other members of the gelsolin family, CapG does not sever preformed actin filaments . This capping activity is regulated by calcium ions and phosphoinositides, which modulate the interaction between CapG and actin filaments .

Biological Significance

CapG is ubiquitously expressed in various tissues and has been implicated in several physiological and pathological processes. It is particularly important in macrophage function, where it regulates cytoplasmic and nuclear structures through potential interactions with actin . Additionally, CapG has been shown to play a role in cell migration and invasion, making it a protein of interest in cancer research .

Clinical Relevance

The expression of CapG has been found to be dysregulated in various cancers, including oral squamous cell carcinoma (OSCC). Studies have shown that CapG is upregulated in OSCC and is associated with advanced clinical stages of the disease . This suggests that CapG could serve as a potential biomarker for cancer progression and a therapeutic target .

Recombinant CapG

Recombinant human CapG is produced using recombinant DNA technology, which involves inserting the gene encoding CapG into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of CapG for research and therapeutic purposes. Recombinant CapG retains the functional properties of the native protein, making it a valuable tool for studying the role of CapG in various biological processes and diseases.

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THE BIOTEK
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