IL-2 Canine
Escherichia Coli.
Interleukin-2, IL-2, T-cell growth factor, TCGF.
Greater than 95.0% as determined by SDS-PAGE.
Description
Interleukin-2 Canine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 136 amino acids and having a molecular mass of 15.6kDa.
IL-2 is purified by proprietary chromatographic techniques.
Product Specs
Interleukin-2, IL-2, T-cell growth factor, TCGF.
Escherichia Coli.
MAPITSSSTK ETEQQMEQLL LDLQLLLNGV NNYENPQLSR MLTFKFYTPK KATEFTHLQC LAEELKNLEE VLGLPQSKNV HLTDTKELIS NMNVTLLKLK GSETSYNCEY DDETATITEF LNKWITFSQS IFSTLT.
Product Science Overview
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system by promoting the growth, differentiation, and survival of certain immune cells. It was initially identified as a T cell growth factor, but its functions extend to other cell types, including B cells, natural killer (NK) cells, and monocytes/macrophages . The recombinant form of canine IL-2 is produced using genetic engineering techniques, primarily in Escherichia coli (E. coli) bacteria .
Recombinant canine IL-2 is typically produced in E. coli as a non-glycosylated polypeptide chain. The production process involves inserting the gene encoding canine IL-2 into E. coli, which then expresses the protein. The recombinant protein is purified using proprietary chromatographic techniques to ensure its purity and functionality .
The recombinant canine IL-2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 136 to 160 amino acids, depending on the specific production method. It has a molecular mass ranging from 15.6 kDa to 18.1 kDa . Some versions of the recombinant protein may include additional tags, such as a His-tag, to facilitate purification .
Recombinant canine IL-2 retains the biological activity of the native protein. It can induce the proliferation of T cells and other immune cells, demonstrating its functional activity . This makes it a valuable tool for research and therapeutic applications, particularly in the field of veterinary medicine.
The primary applications of recombinant canine IL-2 include:
- Research: It is used in various immunological studies to understand the role of IL-2 in the canine immune system. Researchers use it to investigate the mechanisms of T cell activation, proliferation, and differentiation.
- Therapeutics: Recombinant canine IL-2 has potential therapeutic applications in treating immune-related disorders in dogs. It can be used to enhance the immune response in cases of infections, cancer, and other diseases where immune modulation is beneficial.
- Diagnostics: It can be used in diagnostic assays to measure the levels of IL-2 or to assess the functional status of the immune system in dogs.
