CAMK2N1 Mouse
mCaMKIINalpha, calcium/calmodulin-dependent protein kinase II inhibitor alpha, Camk2n1.
Greater than 90.0% as determined by SDS-PAGE.
Description
CAMK2N1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 101 amino acids (1-78 a.a) and having a molecular mass of 10.9kDa.
CAMK2N1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
mCaMKIINalpha, calcium/calmodulin-dependent protein kinase II inhibitor alpha, Camk2n1.
MGSSHHHHHH SSGLVPRGSH MGSMSEVLPY GDEKLSPYGD GGDVGQIFSC RLQDTNNFFG AGQSKRPPKL GQIGRSKRVV IEDDRIDDVL KTMTDKAPPG V.
Product Science Overview
Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) is a multifunctional enzyme that plays a crucial role in various cellular processes, including synaptic plasticity, memory formation, and cardiac function. The inhibition of CaMKII has been a significant area of research due to its implications in various diseases, including cardiac dysfunction and neurological disorders. This article delves into the background of the recombinant form of CaMKII Inhibitor 1 from mice, exploring its structure, function, and potential applications.
CaMKII is a serine/threonine-specific protein kinase regulated by the calcium/calmodulin complex. It is composed of multiple subunits, each containing a catalytic domain, a regulatory domain, and an association domain. The enzyme is activated when calcium-bound calmodulin binds to the regulatory domain, causing a conformational change that exposes the catalytic site.
CaMKII Inhibitor 1 is a peptide that specifically inhibits the activity of CaMKII. It binds to the catalytic domain of CaMKII, preventing its activation by calmodulin. This inhibition is crucial for studying the physiological and pathological roles of CaMKII in various tissues.
The recombinant form of CaMKII Inhibitor 1 is produced using molecular cloning techniques. The gene encoding the inhibitor is inserted into an expression vector, which is then introduced into a host organism, such as Escherichia coli or insect cells. The host cells express the inhibitor protein, which is subsequently purified using chromatographic techniques.
Recombinant expression allows for the production of large quantities of the inhibitor, which is essential for biochemical and pharmacological studies. The recombinant protein is often tagged with a marker, such as His-tag, to facilitate its purification and detection.
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Cardiac Research: CaMKII plays a pivotal role in cardiac function by regulating calcium handling and excitation-contraction coupling. Inhibition of CaMKII has been shown to restore contractility and relaxation in diabetic cardiac muscle, suggesting potential therapeutic applications for CaMKII inhibitors in treating cardiac dysfunction .
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Neurological Studies: CaMKII is highly expressed in the brain and is involved in synaptic plasticity and memory formation. Inhibitors of CaMKII are used to study its role in neurological disorders, such as Alzheimer’s disease and epilepsy.
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Drug Development: The recombinant CaMKII Inhibitor 1 serves as a valuable tool in drug development. By understanding how CaMKII inhibitors interact with the enzyme, researchers can design more effective drugs for treating diseases associated with CaMKII dysregulation.
