CALML5 Human
CLSP, Calmodulin-like skin protein, CALML5, Calmodulin-like protein 5.
Greater than 95.0% as determined by SDS-PAGE.
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Description
CALML5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 2-146) containing 155 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 17.0kDa (calculated).
Product Specs
Calmodulin Like 5, also known as CALML5, belongs to the calmodulin family of calcium-binding proteins. Upon binding calcium, CALML5 undergoes a conformational change. This protein plays a role in the terminal differentiation of keratinocytes. The gene encoding CALML5 is expressed in the epidermis.
Recombinant human CALML5, expressed in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 2-146. It includes a 10-amino acid N-terminal His tag, resulting in a total of 155 amino acids. The calculated molecular mass is 17.0 kDa.
CALML5 is subjected to filtration (0.4 µm) and lyophilization from a solution of 20 mM Tris buffer (pH 7.5) containing 50 mM NaCl and 5% w/v trehalose at a concentration of 0.5 mg/ml.
To prepare a working stock solution, it is recommended to reconstitute the lyophilized pellet with deionized water to an approximate concentration of 0.5 mg/ml. Allow the pellet to dissolve completely. Please note that CALML5 is not sterile. Before using in cell culture, filter the product through an appropriate sterile filter.
Purity exceeds 95.0%, as determined by SDS-PAGE analysis.
CLSP, Calmodulin-like skin protein, CALML5, Calmodulin-like protein 5.
MKHHHHHHAS AGELTPEEEA QYKKAFSAVD TDGNGTINAQ ELGAALKATG KNLSEAQLRK LISEVDSDGD GEISFQEFLT AAKKARAGLE DLQVAFRAFD QDGDGHITVD ELRRAMAGLG QPLPQEELDA MIREADVDQD GRVNYEEFAR MLAQE.
Product Science Overview
CALML5 is a member of the calmodulin family of calcium-binding proteins. These proteins play crucial roles in various cellular processes by binding calcium ions and undergoing conformational changes that allow them to interact with other proteins . The CALML5 protein is specifically expressed in the epidermis, the outermost layer of the skin, and is involved in the terminal differentiation of keratinocytes, which are the predominant cell type in the epidermis .
Recombinant CALML5 refers to the protein that is produced through recombinant DNA technology. This involves inserting the CALML5 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant CALML5 is used in various research applications to study its structure, function, and interactions with other proteins.
Mutations or dysregulation of the CALML5 gene have been associated with certain skin disorders, such as crater-like holes of the optic disc and otomycosis . Understanding the role of CALML5 in skin biology can provide insights into the mechanisms underlying these conditions and potentially lead to the development of targeted therapies.
