C.Albicans Enolase

Candida Albicans Enolase Recombinant
Cat. No.
BT3769
Source
Sf9 insect cells.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Candida Albicans Enolase produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 46kDa. C.Albicans Enolase is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Candida albicans is a diploid fungus capable of existing in both yeast and filamentous forms. It is a common cause of opportunistic infections in humans, particularly oral and genital infections, as well as candidal onychomycosis (nail infection). Systemic infections caused by C. albicans, known as fungemias, pose a significant threat to immunocompromised individuals, such as those with AIDS, undergoing cancer chemotherapy, or who have undergone organ or bone marrow transplantation. C. albicans biofilms can also form on the surface of medical implants. As a commensal organism, C. albicans is part of the normal gut flora residing in the human mouth and gastrointestinal tract. However, an overgrowth of this fungus can lead to candidiasis (also known as candidosis). Candidiasis is frequently observed in immunocompromised individuals, including those infected with HIV. The typical unicellular yeast-like form of C. albicans undergoes a transformation into an invasive, multicellular filamentous form in response to environmental cues, enabling it to infect host tissues. This process is referred to as dimorphism.
Description
Recombinant Candida Albicans Enolase, expressed in SF9 cells, is a glycosylated polypeptide chain with a calculated molecular mass of 46kDa. The protein is produced with a 10xHis tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
C.Albicans Enolase is supplied in a solution of 20mM HEPES buffer at pH 8.0, 200mM NaCl, and 20% glycerol.
Purity
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To maintain product integrity, avoid repeated freeze-thaw cycles.
Source
Sf9 insect cells.

Product Science Overview

Enolase in Candida Albicans

Enolase is an immunodominant antigen in Candida albicans, meaning it is a primary target for the immune response during infection . It is abundantly expressed in the cytoplasm of C. albicans cells and is distributed in a ring-shaped pattern along the cell wall . Interestingly, enolase is also found on the surface of the fungal cells, where it can interact with host immune cells .

Recombinant Enolase

Recombinant enolase refers to the enolase protein that has been cloned, expressed, and purified using recombinant DNA technology. This involves inserting the enolase gene from Candida albicans into a suitable expression vector, which is then introduced into a host organism, such as Escherichia coli, to produce the protein . The recombinant enolase can be tagged with a His-tag to facilitate its purification and detection .

Applications and Research

Recombinant enolase has several important applications in research and medicine:

  1. Diagnostic Marker: Enolase is considered a promising molecular marker for the diagnosis of invasive candidiasis, a severe fungal infection .
  2. Vaccine Development: Due to its immunogenic properties, enolase is being explored as a potential vaccine candidate against Candida infections .
  3. Immune Modulation: Studies have shown that enolase can bind to human CD4+ T cells and modulate immune responses, which could have implications for understanding fungal pathogenesis and developing new therapeutic strategies .

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THE BIOTEK
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