CADM1 Human
Cell Adhesion Molecule 1 Human Recombinant
Cat. No.
BT24892
Source
Escherichia Coli.
Synonyms
Cell Adhesion Molecule 1, Tumor Suppressor In Lung Cancer
1, IGSF4, Spermatogenic Immunoglobulin Superfamily, Immunoglobulin Superfamily,
Member 4, Immunoglobulin Superfamily Member 4, Synaptic Cell Adhesion Molecule,
Nectin-Like Protein 2, Nectin-Like 2, Necl-2, SgIGSF, IGSF4A, SYNCAM, TSLC-1,
TSLC1, NECL2, Immunoglobulin Superfamily, Member 4D Variant 1, Immunoglobulin Superfamily,
Member 4D Variant 2, TSLC1/Nectin-Like 2/IGSF4, Truncated CADM1 Protein,
STSLC-1, SynCAM1, RA175, ST17, BL2, Cell adhesion molecule 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
CADM1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 353 amino acids (45-374 a.a) and
having a molecular mass of 39.4 kDa.
CADM1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Cell Adhesion Molecule 1, also known as CADM1, is a member of the nectin family. This molecule facilitates both homophilic cell-cell adhesion in a calcium-independent manner and heterophilic cell-cell adhesion with CADM3 and PVRL3, also in a calcium-independent manner. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Its interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon gamma (IFN-γ) secretion by CD8+ cells in vitro. This interaction also enhances NK cell-mediated rejection of tumors expressing CADM3 in vivo.
Description
Recombinant human CADM1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 353 amino acids (45-374 a.a.). It has a molecular mass of 39.4 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The CADM1 protein solution is provided at a concentration of 0.25 mg/ml and contains phosphate-buffered saline (pH 7.4), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 85.0% by SDS-PAGE analysis.
Synonyms
Cell Adhesion Molecule 1, Tumor Suppressor In Lung Cancer
1, IGSF4, Spermatogenic Immunoglobulin Superfamily, Immunoglobulin Superfamily,
Member 4, Immunoglobulin Superfamily Member 4, Synaptic Cell Adhesion Molecule,
Nectin-Like Protein 2, Nectin-Like 2, Necl-2, SgIGSF, IGSF4A, SYNCAM, TSLC-1,
TSLC1, NECL2, Immunoglobulin Superfamily, Member 4D Variant 1, Immunoglobulin Superfamily,
Member 4D Variant 2, TSLC1/Nectin-Like 2/IGSF4, Truncated CADM1 Protein,
STSLC-1, SynCAM1, RA175, ST17, BL2, Cell adhesion molecule 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQNLFTKD VTVIEGEVAT ISCQVNKSDD SVIQLLNPNR QTIYFRDFRP
LKDSRFQLLN FSSSELKVSL TNVSISDEGR YFCQLYTDPP QESYTTITVL VPPRNLMIDI QKDTAVEGEE
IEVNCTAMAS KPATTIRWFK GNTELKGKSE VEEWSDMYTV
TSQLMLKVHK EDDGVPVICQ VEHPAVTGNL QTQRYLEVQY KPQVHIQMTY
PLQGLTREGD ALELTCEAIG KPQPVMVTWV RVDDEMPQHA VLSGPNLFIN NLNKTDNGTY RCEASNIVGK
AHSDYMLYVY DPPTTIPPPT TTTTTTTTTT TTILTIITDS RAGEEGSIRA VDH.
Product Science Overview
Structure and Function
CADM1 is a type I transmembrane glycoprotein composed of three extracellular immunoglobulin-like domains, a single transmembrane domain, and a short cytoplasmic tail . The extracellular domains are responsible for mediating homophilic (CADM1-CADM1) and heterophilic (CADM1-CADM3, CADM1-NECTIN3) cell-cell adhesion . This adhesion is crucial for maintaining the structural integrity of tissues and facilitating communication between cells.
Biological Roles
CADM1 is involved in several biological processes, including:
- Cell Recognition: CADM1 enables cells to recognize and adhere to each other, which is essential for tissue formation and maintenance .
- Immune Response: CADM1 interacts with CRTAM (Class I-restricted T cell-associated molecule) to promote natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-γ) secretion by CD8+ T cells . This interaction is vital for the immune system’s ability to target and destroy tumor cells.
- Nervous System: In mast cells, CADM1 may mediate attachment to and communication with nerves, playing a role in the development and survival of mast cells .
Clinical Significance
CADM1 has been implicated in various diseases and conditions:
- Cancer: CADM1 acts as a tumor suppressor in several cancers, including lung cancer, breast carcinoma, and prostate cancer . Its expression is often downregulated in these cancers, leading to increased tumor growth and metastasis.
- Obesity: Genome-wide association studies have identified an association between body mass index (BMI) and loci near the CADM1 gene . Experimental studies have shown that loss of CADM1 can protect mice from obesity by promoting a negative energy balance and weight loss .
- Anorexia Nervosa: A 2019 study revealed an association between CADM1 and anorexia nervosa, suggesting a potential role in the regulation of body weight and eating behavior .
Recombinant CADM1
Recombinant CADM1 is produced using recombinant DNA technology, where the CADM1 gene is cloned and expressed in suitable host cells, such as bacteria or mammalian cells. This allows for the production of large quantities of CADM1 protein for research and therapeutic purposes. Recombinant CADM1 is used in various applications, including:
- Research: Studying the molecular mechanisms of cell adhesion and signaling pathways.
- Therapeutics: Developing potential treatments for diseases where CADM1 is implicated, such as cancer and immune disorders.
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