CACYBP Human

Calcyclin Binding Protein (CACYBP), also known as S100A6 Binding Protein, is a protein encoded by the CACYBP gene in humans. This protein is part of the S100 protein family, which is known for its role in calcium-binding. CACYBP is involved in various cellular processes, including calcium-dependent ubiquitination and proteosomal degradation of target proteins .

CACYBP was first identified and purified over 30 years ago. It was initially studied for its calcium-binding properties and the resultant conformational changes upon binding with calcium ions. The protein’s structure has been extensively analyzed using biophysical methods, revealing its versatility in forming complexes with various ligands .

CACYBP is highly expressed in brain neurons and other tissues. It is localized in the cytoplasm, nucleus, and extracellular exosomes. The protein plays a crucial role in calcium-dependent cellular processes, including cell differentiation, proliferation, and response to stress .

CACYBP serves as an adaptor protein in ubiquitin E3 complexes, participating in the ubiquitin-mediated degradation of β-catenin. This process is essential for regulating protein levels within the cell and maintaining cellular homeostasis. CACYBP also interacts with other proteins, such as SKP1A and SIAH1, forming complexes that are involved in stress responses and other cellular functions .

Altered expression of CACYBP has been observed in various pathological states, including cancers. The protein’s expression levels correlate with the stage and progression of certain diseases, suggesting its potential role as a biomarker. Research continues to explore the mechanisms by which CACYBP contributes to disease development and progression .

Human recombinant CACYBP is produced using recombinant DNA technology, typically expressed in Escherichia coli (E. coli) systems. This recombinant form is used in research to study the protein’s function, interactions, and potential therapeutic applications. The availability of human recombinant CACYBP allows for detailed biochemical and structural analyses, facilitating a deeper understanding of its role in health and disease .