CA8 Human
Description
CA8 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Carbonic Anhydrase 8 (CA8) is a member of the carbonic anhydrase family, which is a group of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. Despite its name, CA8 is unique among carbonic anhydrases because it lacks catalytic activity. This article delves into the background, structure, function, and clinical significance of CA8, particularly focusing on its recombinant form.
CA8 belongs to the alpha-carbonic anhydrase family, which is characterized by a conserved zinc-binding site. However, CA8 is distinct in that it does not possess the typical enzymatic activity associated with other carbonic anhydrases. This is due to the absence of one or more zinc-binding histidine residues, which are crucial for catalytic function .
Despite its lack of enzymatic activity, CA8 plays a significant role in the human body. It is involved in the regulation of intracellular pH and ion transport. CA8 is highly expressed in the cerebellum, where it is believed to play a role in motor coordination and cognitive functions .
Recombinant CA8 is produced using recombinant DNA technology, which involves inserting the CA8 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant form is used in various research applications to study the protein’s structure, function, and potential therapeutic uses .
Mutations in the CA8 gene have been associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome type 3 (CMARQ3). These conditions are characterized by impaired motor coordination, intellectual disability, and balance issues . Understanding the role of CA8 in these disorders can provide insights into potential therapeutic targets.
Research on CA8 has revealed its widespread expression in various tissues, including the cerebellum, cerebrum, lung, liver, salivary gland, and stomach . This broad expression pattern suggests that CA8 may have multiple functions beyond its role in the cerebellum.
Recombinant CA8 is used in research to investigate its role in different physiological processes and its potential involvement in various diseases. Studies have shown that CA8 is involved in the PKC-gamma calcium signaling pathway, which is important for neuronal function .
