CA8 Human, Active
Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP.
Greater than 90.0% as determined by SDS-PAGE.
Description
CA8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-290) and having a molecular mass of 35.5kDa.
CA8 Humanis fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Carbonic Anhydrase VIII, or CA8, was formerly known as CA-related protein because its sequence shares similarities with other identified carbonic anhydrase genes. However, CA8 lacks carbonic anhydrase activity. This protein retains its carbonic anhydrase classification due to consistent sequence homology with other proteins within the carbonic anhydrase family. Mutations in this protein can lead to cerebellar ataxia, mental retardation, and dysequilibrium syndrome, specifically type 3.
Recombinant Human CA8, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 314 amino acids (1-290) and exhibits a molecular weight of 35.5kDa.
A 24 amino acid His-Tag is fused to the N-terminus of Human CA8. Purification is achieved through proprietary chromatographic methods.
The formulation for CA8 Human protein (1mg/ml) consists of 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol and 1mM DTT.
SDS-PAGE analysis confirms purity greater than 90.0%.
Specific activity exceeds 450 pmol/min/ug. This is defined as the enzyme quantity required to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at a pH of 7.5 and a temperature of 37°C.
Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP.
MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE
WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK
SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA
VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW
VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ
Product Science Overview
Carbonic Anhydrase 8 (CA8), also known as Carbonic Anhydrase-Related Protein (CARP), is a member of the carbonic anhydrase family. Despite its name, CA8 does not exhibit the typical enzymatic activity associated with other carbonic anhydrases, which catalyze the reversible hydration of carbon dioxide. Instead, CA8 functions as an allosteric inhibitor of inositol trisphosphate receptor-1 (ITPR1), playing a crucial role in regulating neuronal intracellular calcium release .
The CA8 gene is located on chromosome 8 in humans and encodes a protein that consists of 314 amino acids. The recombinant form of CA8 is typically produced in Escherichia coli (E. coli) and is fused to a 24 amino acid His-tag at the N-terminus to facilitate purification. The molecular mass of the recombinant protein is approximately 35.5 kDa .
Although CA8 lacks carbonic anhydrase activity, it is involved in important biological processes. CA8 acts as an allosteric inhibitor of ITPR1, which is essential for the regulation of calcium signaling in neurons. This regulation is critical for maintaining proper neuronal function and intracellular calcium homeostasis .
Mutations in the CA8 gene have been associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome type 3 (CMARQ3). These conditions are characterized by impaired motor coordination, intellectual disability, and balance issues. Understanding the role of CA8 in these disorders can provide insights into potential therapeutic targets for treating such conditions .
The recombinant human CA8 protein is produced in E. coli and purified using proprietary chromatographic techniques. The protein is supplied as a sterile, filtered, colorless solution containing 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, and 20% glycerol. The purity of the recombinant CA8 protein is greater than 90%, as determined by SDS-PAGE .
For short-term storage, the recombinant CA8 protein can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability and activity .
