C10ORF54 Mouse

Chromosome 10 Open Reading Frame 54 Mouse Recombinant
Cat. No.
BT8919
Source
Sf9, Insect cells.
Synonyms

GI24, Platelet receptor Gi24, VSIR, V-Set Immunoregulatory Receptor, SISP1, GI-24, B7-H5, Stress Induced Secreted Protein 1. VISTA.

Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 

Shipped with Ice Packs
In Stock

Description

C10ORF54 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 167 amino acids (33-191a.a.) and having a molecular mass of 18.8kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
C10ORF54 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chromosome 10 Open Reading Frame 54, also referred to as C10ORF54, belongs to the immunoglobulin superfamily. This transmembrane molecule is found on the surface of bone embryonic stem cells (ESCs) and tumor cells. C10ORF54 plays a role in ESC differentiation and enhances BMP4-induced signaling in ESCs. Interestingly, its expression is downregulated after exposure to BMP4.
Description
Produced in Sf9 insect cells, C10ORF54 is a single, glycosylated polypeptide chain with a molecular weight of 18.8 kDa (appears at approximately 28-40 kDa on SDS-PAGE). It consists of 167 amino acids (33-191a.a.) and features an 8 amino acid His tag at the C-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The product is a clear and sterile solution without any color.
Formulation
The C10ORF54 protein solution is provided at a concentration of 1 mg/ml and contains Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept at 4°C. For longer periods, it's recommended to store the product frozen at -20°C. To ensure stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). It is crucial to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
Analysis by SDS-PAGE confirms a purity level exceeding 90.0%.
Synonyms

GI24, Platelet receptor Gi24, VSIR, V-Set Immunoregulatory Receptor, SISP1, GI-24, B7-H5, Stress Induced Secreted Protein 1. VISTA.

Source
Sf9, Insect cells.
Amino Acid Sequence
FKVTTPYSLY VCPEGQNATL TCRILGPVSK GHDVTIYKTW YLSSRGEVQM CKEHRPIRNF TLQHLQHHGS HLKANASHDQ PQKHGLELAS DHHGNFSITL RNVTPRDSGL YCCLVIELKN HHPEQRFYGS MELQVQAGKG SGSTCMASNE QDSDSITAAL EHHHHHH.

Product Science Overview

Gene and Protein Structure

The C10orf54 gene consists of several exons and introns, which are segments of DNA that code for the protein and non-coding regions, respectively. The open reading frame (ORF) refers to the portion of the gene that is translated into the protein. The protein encoded by C10orf54 is composed of a specific sequence of amino acids, which determines its structure and function.

Function and Biological Significance

The exact function of the C10orf54 protein in mice is still under investigation. However, it is believed to play a role in various cellular processes, including cell signaling, growth, and differentiation. Studies have shown that proteins encoded by similar genes in other organisms are involved in important biological pathways, such as immune response and cell cycle regulation.

Recombinant Protein Production

Recombinant C10orf54 protein is produced by inserting the gene into an expression vector, which is then introduced into a host cell, such as bacteria or yeast. The host cells are cultured under specific conditions to express the protein, which is then purified for further use. This recombinant protein can be used in various research applications, including studying its function, interactions with other proteins, and potential therapeutic uses.

Research and Applications

Research on C10orf54 and its recombinant protein is ongoing, with scientists exploring its potential roles in health and disease. The recombinant protein can be used in various experimental setups to understand its function and interactions. Additionally, it may have potential therapeutic applications, such as in the development of drugs targeting specific pathways involving C10orf54.

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