C-10 Rat
C-10 Rat Recombinant (CCL6)
Cat. No.
BT9922
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine (C-C motif) ligand 6.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
C-10 Rat Recombinant produced in E.Coli is a signle, non-glycosylated, polypeptide chain containing 94 amino acids and having a total molecular mass of 10.4kDa.
C-10 is purified by proprietary chromatographic techniques.
C-10 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that is found exclusively in rodents. In mice, CCL6 is produced by cells of the neutrophil and macrophage lineages. Its production significantly increases under conditions that promote myeloid cell differentiation, such as stimulation with the cytokine GM-CSF in bone marrow cultures. Low levels of CCL6 expression are also observed in certain myeloid cell lines, including the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D. This expression can be significantly enhanced by GM-CSF treatment. Conversely, CCL6 expression is substantially reduced in activated T cell lines. In the mouse lung, CCL6 can be induced by the cytokine interleukin 13. The gene encoding mouse CCL6 is located on chromosome 11. The chemokine receptor CCR1 is thought to be the primary cell surface receptor for CCL6.
Description
Recombinant rat C-10, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 94 amino acids. It has a molecular weight of 10.4 kDa. C-10 is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, white, lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 μm filtered solution at a concentration of 1.0 mg/ml in 1x phosphate-buffered saline (PBS), pH 7.4.
Solubility
To reconstitute lyophilized C-10, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 μg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized C-10 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, C-10 can be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. To enhance stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Exceeds 95.0% purity, as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis, and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of C-10 is assessed by its ability to induce chemotaxis in human CCR1-transfected BaF3 mouse pro-B cells. The concentration range used for this assay is 0.05-0.25 μg/ml.
Synonyms
Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine (C-C motif) ligand 6.
Source
Escherichia Coli.
Amino Acid Sequence
GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA.
Product Science Overview
Structure and Production
Expression and Function
In rodents, CCL6 is expressed in cells from neutrophil and macrophage lineages. Its expression can be significantly induced under conditions favorable for myeloid cell differentiation . For instance, in mice, CCL6 is highly expressed in bone marrow cultures stimulated with the cytokine GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) . Additionally, low levels of gene expression are observed in certain myeloid-origin cell lines, such as DA3, 32D cl3, and the macrophage cell line P388D . However, in activated T cell lines, the expression of CCL6 is greatly reduced .
Biological Activity
Applications
Storage and Stability
Lyophilized C-10 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. To ensure stability, it is recommended to add a carrier protein, such as 0.1% HSA or BSA, and to avoid freeze-thaw cycles .
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