C-10 Mouse

C-10 Mouse Recombinant (CCL6)
Cat. No.
BT9845
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine (C-C motif) ligand 6.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

C-10 Mouse Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 95 amino acids and having a molecular mass of 10.7kDa.
The CCL6 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g., the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Description
C-10 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 95 amino acids and having a molecular mass of 10.7kDa. CCL6 is purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder
Formulation
CCL6 Mouse was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized C10 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized C10 protein, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution, CCL6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The biological activity was determined by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells using a concentration range of 0.05-0.25µg/ml.
Synonyms
Small inducible cytokine A6, CCL6, C10 protein, c10, MRP-1, Scya6, chemokine (C-C motif) ligand 6.
Source
Escherichia Coli.
Amino Acid Sequence

GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA.

Product Science Overview

Discovery and Expression

CCL6 was initially identified as a transcript induced in bone marrow cells upon stimulation with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) . It is highly expressed in bone marrow cultures stimulated with GM-CSF and can be significantly induced under conditions favorable for myeloid cell differentiation . Additionally, CCL6 expression is upregulated by Interleukin-4 (IL-4) in mouse macrophages and Stem Cell Factor (SCF) in eosinophils .

Biological Functions

CCL6 is involved in various biological processes, particularly in inflammation and immune response. It chemoattracts human CCR1 transfected BaF3 cells in a dose-dependent manner . This chemokine is constitutively expressed by murine small intestinal and colonic epithelial cells, where it binds to a subset of the intestinal microflora and displays antibacterial activity .

Role in Diseases

CCL6 has been implicated in several remodeling diseases, including:

  • IL-13-induced alveolar remodeling
  • Bleomycin-induced pulmonary fibrosis
  • Skin wound healing
  • Inflammatory demyelinating disorders
Molecular Characteristics

The recombinant mouse CCL6 protein is produced in E. coli and consists of 95 amino acids with a predicted molecular mass of approximately 10.7 kDa . The protein migrates at approximately 14 kDa in both DTT-reducing and non-reducing conditions by SDS-PAGE . It is highly purified, with a purity greater than 97% as determined by SDS-PAGE under reducing conditions .

Storage and Handling

Recombinant mouse CCL6 can be stored at various temperatures depending on the duration:

  • 2°C to 8°C for up to 2 weeks
  • -20°C for up to six months
  • -70°C or colder for long-term storage

For maximum results, it is recommended to quick spin the vial prior to opening and to aliquot and store the protein at -20°C or colder. Stock solutions can be prepared at 50-100 µg/mL in an appropriate sterile buffer .

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Source
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