Borrelia p41, Sf9
Description
Borrelia p41 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Borrelia burgdorferi is a species of bacteria belonging to the genus Borrelia, which is part of the spirochete phylum. This bacterium is primarily known for causing Lyme disease, a zoonotic, vector-borne disease transmitted mainly by ticks. Borrelia burgdorferi, along with Borrelia afzelii and Borrelia garinii, are the main species responsible for Lyme disease .
Members of the Borrelia genus have a unique genetic structure. They possess a linear chromosome approximately 900 kilobase pairs (kbp) in length, along with an array of both linear and circular plasmids ranging from 5 to 220 kbp. These plasmids are atypical compared to most bacterial plasmids, as they contain many paralogous sequences, a large number of pseudogenes, and, in some cases, essential genes. Some plasmids also exhibit features suggesting they are prophages .
The p41 protein of Borrelia burgdorferi is an immunodominant antigen, meaning it is a primary target of the immune response during infection. This protein is often used in diagnostic assays for Lyme disease due to its strong immunogenic properties.
The recombinant Borrelia burgdorferi p41 protein produced in Sf9 insect cells is a glycosylated polypeptide chain with a calculated molecular mass of 36,578 Daltons. This recombinant protein is expressed with a 6x His tag at the N-terminus and purified using proprietary chromatographic techniques .
Source and Production
- Source: The recombinant p41 protein is produced in Sf9 insect cells.
- Expression: The protein is expressed with a 6x His tag at the N-terminus.
- Purification: Purification is achieved through proprietary chromatographic techniques .
Physical and Chemical Properties
- Physical Appearance: The recombinant protein is supplied as a sterile filtered clear solution.
- Formulation: It is formulated in 20mM HEPES buffer (pH 7.5), 0.01mM EDTA, and 0.02% SDS.
- Stability: The protein should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. Multiple freeze-thaw cycles should be avoided to maintain stability .
Applications
The recombinant Borrelia burgdorferi p41 protein is primarily used in laboratory research, particularly in Western blot assays with Lyme positive plasma .
