Borrelia p100
Description
Borrelia p100 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Borrelia burgdorferi is a spirochete bacterium responsible for Lyme disease, a tick-borne illness that affects humans and animals. The bacterium is transmitted through the bite of infected Ixodes ticks. Among the various proteins expressed by Borrelia burgdorferi, the p100 protein, also known as p83, is of particular interest due to its role in the immune response and its potential use in diagnostic assays.
The p100 protein is a high-molecular-weight protein that is expressed on the surface of Borrelia burgdorferi. It is one of the immunodominant antigens, meaning it elicits a strong immune response in infected individuals. The protein has a calculated molecular mass of approximately 77,813 Daltons and is glycosylated, which means it has sugar molecules attached to it .
Recombinant p100 protein is produced using genetic engineering techniques. The gene encoding the p100 protein is cloned into an expression vector, which is then introduced into a host cell, such as the SF9 insect cell line. The host cells produce the p100 protein, which is then purified using chromatographic techniques. The recombinant p100 protein is often tagged with a His-tag at the N-terminus to facilitate purification .
The p100 protein is used in serological assays to diagnose Lyme disease. Serological assays detect antibodies produced by the immune system in response to infection. The p100 protein is included in enzyme-linked immunosorbent assays (ELISAs) and immunoblots as an antigen to capture antibodies from patient samples. The presence of antibodies against p100 in a patient’s serum indicates exposure to Borrelia burgdorferi .
- High Sensitivity and Specificity: Recombinant p100 protein provides high sensitivity and specificity in serological assays, making it a reliable marker for Lyme disease diagnosis.
- Consistency: Recombinant production ensures a consistent supply of high-quality protein, reducing variability in diagnostic tests.
- Ease of Purification: The His-tag facilitates easy purification of the recombinant protein, ensuring high purity and yield .
