Borrelia p41

Borrelia Burgdorferi p41 Recombinant
Cat. No.
BT29684
Source
Escherichia Coli.
Synonyms
Appearance
Purity
Protein is >90% pure as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The E.Coli derived recombinant protein contains the p41 immunodominant regions, 158-296 amino acids. Borrelia p41 is fused to 6xHis tag at N-terminal and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Borrelia is a genus of bacteria belonging to the spirochete phylum. These bacteria are responsible for causing borreliosis, a zoonotic disease transmitted primarily through ticks and, in some cases, lice, depending on the specific species. Among the 36 recognized Borrelia species, 12 are known to cause Lyme disease, also known as borreliosis, and are spread through tick bites. The primary species implicated in Lyme disease include Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. Members of the Borrelia genus possess a linear chromosome approximately 900 kbp in length, along with a multitude of linear and circular plasmids ranging in size from 5 to 220 kbp. These plasmids are unique compared to typical bacterial plasmids due to their high content of paralogous sequences, numerous pseudogenes, and, in certain instances, essential genes. Additionally, several plasmids exhibit characteristics suggesting they may be prophages.
Description
The recombinant Borrelia p41 protein, derived from E. coli, encompasses the immunodominant regions of the p41 protein, specifically amino acids 158-296. This protein is further modified with a 6xHis tag fused to its N-terminus and purified using proprietary chromatographic methods.
Purity
The purity of the protein exceeds 90% as determined by SDS-PAGE analysis.
Formulation
The protein is supplied in a solution containing 25mM glycine at pH 9.6 and 50% glycerol.
Stability
For optimal stability, Borrelia p41 should be stored below -18°C. While it can withstand temperatures of 4°C for up to one week, it is recommended to avoid repeated freeze-thaw cycles.
Source
Escherichia Coli.
Purification Method

Purified by proprietary chromatographic technique.

Product Science Overview

Introduction

Borrelia burgdorferi is a spirochete bacterium primarily known for causing Lyme disease, a vector-borne illness transmitted to humans through the bite of infected ticks, particularly those of the Ixodes ricinus complex . The bacterium has a complex genome and expresses various proteins that play crucial roles in its pathogenicity and immune evasion.

Borrelia Burgdorferi p41 Protein

One of the significant proteins expressed by Borrelia burgdorferi is the p41 protein, also known as flagellin. This protein is a component of the bacterium’s flagellum, which is essential for its motility. The p41 protein is highly immunogenic, meaning it can elicit a strong immune response in the host. This makes it a valuable target for diagnostic assays and vaccine development .

Recombinant p41 Protein

Recombinant p41 protein refers to the p41 protein that has been produced using recombinant DNA technology. This involves inserting the gene encoding the p41 protein into an expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant p41 protein retains the immunogenic properties of the native protein, making it useful for various applications in research and diagnostics .

Applications in Diagnostics

The recombinant p41 protein is widely used in serological assays for the diagnosis of Lyme disease. These assays detect antibodies against Borrelia burgdorferi in the patient’s serum. The presence of antibodies against the p41 protein indicates exposure to the bacterium and can help confirm a diagnosis of Lyme disease . The use of recombinant p41 protein in these assays improves their sensitivity and specificity, leading to more accurate diagnostic results .

Research and Vaccine Development

In addition to its diagnostic applications, the recombinant p41 protein is also used in research to study the immune response to Borrelia burgdorferi. Understanding how the immune system recognizes and responds to the p41 protein can provide insights into the pathogenesis of Lyme disease and aid in the development of effective vaccines . Researchers are exploring the potential of using the recombinant p41 protein as a component of a Lyme disease vaccine, aiming to elicit a protective immune response in vaccinated individuals .

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