Borrelia p28
Description
Borrelia Burgdorferi p28 Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 27kDa. Borrelia p28 is expressed with a 10xHis tag and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Borrelia burgdorferi is a spirochete bacterium responsible for Lyme disease, a tick-borne illness prevalent in the northern hemisphere. The bacterium is part of the Borrelia burgdorferi sensu lato complex, which includes several species pathogenic to humans. Among the various proteins expressed by Borrelia burgdorferi, the p28 protein has garnered significant attention for its role in the bacterium’s life cycle and its potential as a diagnostic marker.
Lyme disease is the most common tick-borne infectious disease in the northern hemisphere. It is transmitted to humans through the bite of infected black-legged ticks. The early phase of the infection is typically associated with a characteristic skin lesion known as erythema migrans. If left untreated, the infection can disseminate to other parts of the body, leading to more severe symptoms affecting the joints, heart, and nervous system .
The p28 protein is one of the outer surface proteins of Borrelia burgdorferi. It plays a crucial role in the bacterium’s ability to evade the host’s immune system and establish infection. The protein is expressed during different stages of the bacterium’s life cycle, making it a valuable target for diagnostic assays .
Recombinant p28 protein refers to the p28 protein that has been produced using recombinant DNA technology. This involves inserting the gene encoding the p28 protein into a suitable expression system, such as bacteria or yeast, to produce large quantities of the protein. Recombinant p28 protein is used in various research and diagnostic applications, including the development of serological tests for Lyme disease .
The detection of antibodies against Borrelia burgdorferi proteins, including p28, is a common method for diagnosing Lyme disease. Serological tests, such as enzyme-linked immunosorbent assays (ELISAs) and immunoblots, often use recombinant p28 protein as an antigen to capture specific antibodies from patient samples. The presence of these antibodies indicates exposure to the bacterium and can aid in the diagnosis of Lyme disease .
