Borrelia Bavariensis DbpA
Decorin binding protein A, dbpA.
Description
Recombinant Borrelia Bavariensis DbpA produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 18kDa.
Borrelia Bavariensis DbpA is expressed with a 10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Decorin binding protein A, dbpA.
Product Science Overview
Borrelia bavariensis is a species of spirochete bacteria within the Borrelia burgdorferi sensu lato complex, which is known to cause Lyme disease in humans. This bacterium is primarily transmitted by ticks, specifically Ixodes persulcatus in Asia and Ixodes ricinus in Europe . Borrelia bavariensis was elevated to species level in 2009, distinguishing it from its closely related species, Borrelia garinii .
Decorin binding protein A (DbpA), also known as Osp17 or p17, is a surface protein expressed by Borrelia bavariensis during the mammalian phase of infection . DbpA plays a crucial role in the pathogenesis of Lyme disease by binding to decorin, a collagen-associated proteoglycan found in the extracellular matrix of the host . This interaction facilitates the adherence of the bacteria to host tissues, aiding in colonization and infection.
Recombinant Borrelia bavariensis DbpA is produced using Escherichia coli (E. coli) as a host organism . The recombinant protein is a non-glycosylated polypeptide chain with a calculated molecular mass of approximately 18 kDa . It is expressed with a 10x His tag at the N-terminus, which allows for purification using chromatographic techniques .
The recombinant DbpA protein is supplied in a sterile, filtered clear solution and formulated in 20mM HEPES buffer (pH 7.6), 250mM NaCl, and 20% glycerol . It is typically stored at 4°C for short-term use or frozen at -20°C for long-term storage to maintain stability .
Recombinant Borrelia bavariensis DbpA has several applications in laboratory research, particularly in the study of Lyme disease. It is used in Western blot assays with patient samples to detect the presence of antibodies against DbpA . Additionally, it is employed in immunodot tests to differentiate between Lyme disease-positive and negative patient samples .
The protein binds to both IgG and IgM-type human antibodies, making it a valuable tool for immunological studies . These applications help researchers understand the immune response to Borrelia bavariensis infection and develop diagnostic tools for Lyme disease.
