BMPR1A Human, CHO
CHO cells.
BMPR-1A, BMP-R1A, BMPR1A, BMR1A, CD292, CD-292, Serine/threonine-protein kinase receptor R5, SKR5, ALK-3, ACVRLK3, EC 2.7.11.30, CD292 antigen.
Greater than 95.0% as determined by SDS-PAGE.
Description
Bone Morphogenetic Protein Receptor-1A Human Recombinant produced in CHO cells is a glycosylated homodimer chain containing 2x362 amino acids and having a total molecular mass of 80.8kDa.
BMPR1A is purified by proprietary chromatographic techniques.
Product Specs
The bone morphogenetic protein (BMP) receptors are transmembrane serine/threonine kinases. This family includes type I receptors BMPR1A and BMPR1B, and the type II receptor BMPR2. These receptors are closely related to ACVR1 and ACVR2 receptors. Ligands for these receptors belong to the TGF-beta superfamily. TGF-betas signal through heteromeric complexes formed with two types of serine (threonine) kinase receptors: type I receptors (approximately 50-55 kD) and type II receptors (approximately 70-80 kD). Type II receptors can bind ligands independently but require type I receptors for signaling. Conversely, type I receptors need their corresponding type II receptors for ligand binding.
Recombinant Human Bone Morphogenetic Protein Receptor-1A, produced in CHO cells, is a glycosylated homodimer. It consists of two chains, each containing 362 amino acids, resulting in a total molecular mass of 80.8kDa.
Purification of BMPR1A is achieved using proprietary chromatographic techniques.
The protein solution undergoes sterile filtration (0.2µm) and is then lyophilized in a buffer containing PBS.
For reconstitution, it is recommended to dissolve the lyophilized BMPR1A in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized BMPR1A remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, BMPR1A should be stored at 4°C for 2-7 days. For future use, store at -18°C.
Avoid repeated freeze-thaw cycles.
The purity is determined to be greater than 95.0% as assessed by SDS-PAGE.
The ED50, determined by measuring the inhibition of human BMP-4-induced alkaline phosphatase production in ATDC5 cells, is 120ng/ml. This corresponds to a specific activity of 8.3 x 10^3 units/mg.
BMPR-1A, BMP-R1A, BMPR1A, BMR1A, CD292, CD-292, Serine/threonine-protein kinase receptor R5, SKR5, ALK-3, ACVRLK3, EC 2.7.11.30, CD292 antigen.
CHO cells.
QNLDSMLHGT GMKSDSDQKK SENGVTLAPE DTLPFLKCYC SGHCPDDAIN NTCITNGHCF AIIEEDDQGE TTLASGCMKY EGSDFQCKDS PKAQLRRTIE CCRTNLCNQY LQPTLPPVVI GPFFDGSIRI EGRMDDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK.
Product Science Overview
Bone Morphogenetic Protein Receptor-1A (BMPR1A) is a protein encoded by the BMPR1A gene. This receptor is part of the transforming growth factor-beta (TGF-β) superfamily, which plays a crucial role in various cellular processes, including growth, differentiation, and apoptosis . BMPR1A is a type I receptor that binds to bone morphogenetic proteins (BMPs), which are involved in bone and cartilage development .
BMPR1A is a transmembrane serine/threonine kinase receptor. It forms a receptor complex with type II receptors upon ligand binding. This complex then phosphorylates intracellular SMAD proteins, which transduce the signal to the nucleus, leading to the regulation of target gene expression . The receptor is essential for the proper functioning of BMP signaling pathways, which are critical for skeletal development and homeostasis .
BMPR1A can be expressed in various systems, including Chinese Hamster Ovary (CHO) cells. The recombinant human BMPR1A produced in CHO cells is biologically active and is used in various research applications, such as kinase assays . The use of CHO cells for recombinant protein production is advantageous due to their ability to perform post-translational modifications similar to those in human cells .
Mutations in the BMPR1A gene are associated with several disorders, including juvenile polyposis syndrome and hereditary mixed polyposis syndrome . These conditions are characterized by the development of numerous polyps in the gastrointestinal tract, which can increase the risk of colorectal cancer .
