BMP4 Human, CHO
CHO cells.
BMP4, ZYME, BMP2B, BMP2B1.
Greater than 95.0% as determined by SDS-PAGE.
Description
Bone Morphogenetic protein-4 Active Human Recombinant produced in CHO cells is a glycosylated homodimer chain containing 2x116 amino acids and having a total molecular mass of 26.2kDa.
BMP4 is purified by proprietary chromatographic techniques.
Product Specs
This gene encodes a protein that belongs to the bone morphogenetic protein family, which is part of the transforming growth factor-beta superfamily. This superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were initially discovered due to the ability of demineralized bone extract to stimulate endochondral osteogenesis in vivo at an extraskeletal site. This specific family member plays a crucial role in initiating endochondral bone formation in humans. Reduced expression of this protein has been linked to various bone diseases, including the inherited disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing has been observed in the 5' untranslated region of this gene, resulting in three identified variants that all encode the same protein.
Recombinant Human Bone Morphogenetic protein-4, produced in CHO cells, is a glycosylated homodimer chain comprising 2x116 amino acids. It has a total molecular mass of 26.2kDa. BMP4 undergoes purification using proprietary chromatographic techniques.
The protein was lyophilized from a sterile (0.2µm) filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
For reconstitution of lyophilized BMP4, sterile 10mM HCl is recommended at a concentration of 0.1 mg/ml. This can be further diluted in other aqueous solutions.
Lyophilized BMP4 remains stable at room temperature for up to 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store BMP4 at 4°C for 2-7 days. For long-term storage, keep it below -18°C. It is important to avoid repeated freeze-thaw cycles.
The purity is determined to be greater than 95.0% by SDS-PAGE analysis.
The ED50, determined by measuring Alkaline phosphatase activity induced in ATDC-5 cells, is 15ng/ml. This corresponds to a specific activity of 6.7 x 10^4 units/mg.
BMP4, ZYME, BMP2B, BMP2B1.
CHO cells.
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Product Science Overview
Bone Morphogenetic Protein-4 (BMP-4) is a member of the bone morphogenetic protein family, which is part of the transforming growth factor-beta superfamily. This superfamily includes large families of growth and differentiation factors. BMP-4 plays a crucial role in bone and cartilage development, specifically in the onset of endochondral bone formation in humans .
Bone Morphogenetic Protein-4 Active (Human Recombinant, CHO) is produced in Chinese Hamster Ovary (CHO) cells. The recombinant protein is a glycosylated homodimer chain containing 2x116 amino acids, with a total molecular mass of 26.2 kDa . The production in CHO cells ensures proper glycosylation, which contributes to the stability and biological activity of the protein .
BMP-4 is known for its ability to induce endochondral osteogenesis in vivo at extraskeletal sites. This property was initially identified through the ability of demineralized bone extract to induce bone formation . The protein stimulates the differentiation of mesenchymal stem cells into osteoblasts, which are essential for bone formation and repair .
BMP-4 is highly conserved evolutionarily and is involved in various developmental processes. During embryonic development, BMP-4 is found in the ventral marginal zone and plays a role in the formation of the dorsal-ventral axis. It is also involved in the development of the eye, heart, blood, and otic vesicle . Additionally, BMP-4 helps in the patterning of the developing head by inducing apoptosis of neural crest cells .
BMP-4 has significant clinical relevance due to its role in bone formation and repair. A reduction in BMP-4 expression has been associated with various bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva . The recombinant form of BMP-4 is used in laboratory research to study its effects on cell differentiation and bone formation .
The lyophilized form of BMP-4 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, BMP-4 should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. It is important to prevent freeze-thaw cycles to maintain the protein’s stability .
