BMP 4 Human

Bone Morphogenetic Protein-4 Human Recombinant
Cat. No.
BT16424
Source
Escherichia Coli.
Synonyms
BMP4, ZYME, BMP2B, BMP2B1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13kDa.
The BMP-4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
This gene encodes a protein that belongs to the bone morphogenetic protein family, a member of the transforming growth factor-beta superfamily. This superfamily encompasses large groups of growth and differentiation factors. The initial identification of bone morphogenetic proteins stemmed from their ability within demineralized bone extract to stimulate endochondral osteogenesis in vivo at extraskeletal locations. This specific family member plays a crucial role in initiating endochondral bone formation in humans. Reduced expression of this protein has been linked to various bone diseases, including the inherited disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing has been observed in the 5' untranslated region of this gene, resulting in three identified variants that all encode the same protein.
Description
Recombinant Human Bone Morphogenetic Protein-4, produced in E. coli, is a monomeric, non-glycosylated polypeptide chain consisting of 116 amino acids with a molecular weight of 13 kDa. The purification of BMP-4 is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
BMP-4 was lyophilized from a 0.2µm filtered solution concentrated to 1mg/ml in 20mM Na₂CO₃ buffer at a pH of 9.0.
Solubility
For reconstitution of the lyophilized Bone Morphogenetic Protein-4, it is recommended to use sterile 18M-cm H₂O at a concentration of at least 100µg/ml. This solution can be further diluted into other aqueous solutions.
Stability
Lyophilized Bone Morphogenetic Protein-4 remains stable at room temperature for a period of 3 weeks. However, for extended storage, it should be kept desiccated at a temperature below -18°C. After reconstitution, BMP4 should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 95.0% based on the following analyses: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
BMP4, ZYME, BMP2B, BMP2B1.
Source
Escherichia Coli.
Amino Acid Sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.

Product Science Overview

Introduction

Bone Morphogenetic Protein-4 (BMP-4) is a member of the bone morphogenetic protein family, which is part of the transforming growth factor-beta (TGF-β) superfamily. This family includes a large number of growth and differentiation factors that play crucial roles in various biological processes, including embryonic development, bone and cartilage formation, and tissue repair .

Discovery

Bone morphogenetic proteins were originally identified by their ability to induce endochondral osteogenesis in vivo at extraskeletal sites. This discovery highlighted their potential in bone and cartilage development and repair .

Structure and Function

BMP-4 is a polypeptide that functions as a disulfide-linked homodimer. It is highly conserved evolutionarily and is involved in several critical developmental processes. In humans, BMP-4 is encoded by the BMP4 gene located on chromosome 14q22-q23 .

BMP-4 plays a significant role in:

  • Bone and Cartilage Development: It is involved in the onset of endochondral bone formation, tooth and limb development, and fracture repair .
  • Embryonic Development: BMP-4 is crucial in establishing the dorsal-ventral axis in early embryonic development. It also helps in the patterning of the developing neural tube and induces the formation of epidermis rather than neural tissue .
  • Muscle Development and Bone Mineralization: BMP-4 is involved in muscle development and bone mineralization, as well as the development of the ureteric bud .
Recombinant Human BMP-4

Recombinant human BMP-4 is produced using advanced biotechnological methods. It is typically expressed in mammalian cells to ensure proper folding and post-translational modifications. The recombinant protein is used in various research and clinical applications due to its high purity and bioactivity .

Applications

Recombinant human BMP-4 is widely used in:

  • Bone and Cartilage Repair: It is used in regenerative medicine to promote bone and cartilage repair.
  • Developmental Biology Research: BMP-4 is used to study various aspects of embryonic development and tissue differentiation.
  • Stem Cell Research: It is used to induce the differentiation of stem cells into specific cell types, such as osteoblasts and chondrocytes .

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