BIN2 Human
Bridging Integrator 2, Breast Cancer-Associated Protein 1, Breast Cancer Associated Protein BRAP1, BRAP-1, BRAP1, BIN2.
Greater than 80.0% as determined by SDS-PAGE.
Description
BIN2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (1-597 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 606 amino acids and having a molecular mass of 66.1kDa.
BIN2 shows multiple bands between 70-100kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Bridging Integrator 2, Breast Cancer-Associated Protein 1, Breast Cancer Associated Protein BRAP1, BRAP-1, BRAP1, BIN2.
ADPMRGMPGA RTSSSGASEN HRARGQGGGP QGVGRMAEGK AGGAAGLFAK QVQKKFSRAQ EKVLQKLGKA VETKDERFEQ SASNFYQQQA EGHKLYKDLK NFLSAVKVMH ESSKRVSETL QEIYSSEWDG HEELKAIVWN NDLLWEDYEE KLADQAVRTM EIYVAQFSEI KERIAKRGRK LVDYDSARHH LEAVQNAKKK DEAKTAKAEE EFNKAQTVFE DLNQELLEEL PILYNSRIGC YVTIFQNISN LRDVFYREMS KLNHNLYEVM SKLEKQHSNK VFVVKGLSSS SRRSLVISPP VRTATVSSPL TSPTSPSTLS LKSESESVSA TEDLAPDAAQ GEDNSEIKEL LEEEEIEKEG SEASSSEEDE PLPACNGPAQ AQPSPTTERA KSQEEVLPSS TTPSPGGALS PSGQPSSSAT EVVLRTRTAS EGSEQPKKRA SIQRTSAPPS RPPPPRATAS PRPSSGNIPS SPTASGGGSP TSPRASLGTG TASPRTSLEV SPNPEPPEKP VRTPEAKENE NIHNQNPEEL CTSPTLMTSQ VASEPGEAKK MEDKEKDNKL ISANSSEGQD QLQVSMVPEN NNLTAPEPQE EVSTSENPQL HHHHHH
Product Science Overview
BIN2 contains a single BAR domain, which is crucial for its membrane-binding and bending properties . This protein is highly expressed in various hematopoietic tissues, including peripheral blood, thymus, colon, and placenta . The BAR domain allows BIN2 to associate with actin-rich structures on the plasma membrane, influencing cellular dynamics .
BIN2 is involved in several critical cellular functions:
- Membrane Sculpting: BIN2’s BAR domain enables it to bind and bend membranes, playing a role in membrane remodeling processes .
- Cell Motility: BIN2 influences cell movement by interacting with actin structures .
- Phagocytosis: BIN2 affects the process of phagocytosis, where cells engulf and digest particles .
- Podosome Formation: BIN2 is involved in the formation and dynamics of podosomes, which are actin-rich structures important for cell adhesion and migration .
The expression and activity of BIN2 are regulated by various factors, including its interaction with other proteins and its localization within the cell . Overexpression of BIN2 can lead to decreased phagocytosis and increased podosome density and dynamics, while knockdown of BIN2 results in decreased cell migration and increased phagocytosis .
Understanding the functions and regulatory mechanisms of BIN2 is crucial for comprehending the behavior of leukocytes under physiological and pathological conditions . Further research on BIN2 may provide insights into its potential roles in diseases and its applications in therapeutic interventions.
For more detailed information, you can refer to the PLOS ONE article and other sources .
