BDH2 Human

BDH2 is a single, non-glycosylated polypeptide chain composed of 265 amino acids, with a molecular mass of approximately 28.8 kDa . The recombinant form of this enzyme is typically expressed in Escherichia coli (E. coli) and is purified using proprietary chromatographic techniques . The enzyme is often tagged with a His-tag at the N-terminus to facilitate purification .

BDH2 mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin . This function is crucial for iron assimilation and homeostasis, as 2,5-DHBA associates with lipocalin 2 (LCN2), playing a key role in iron metabolism . Additionally, BDH2 acts as a 3-hydroxybutyrate dehydrogenase, converting 3-hydroxybutyrate into acetoacetate, which is an essential step in ketone body metabolism .

The recombinant form of BDH2 is used extensively in biochemical research to study its role in ketone body metabolism and iron homeostasis . It is also utilized in high-throughput screening assays and other experimental setups to understand its enzymatic properties and potential therapeutic applications .

BDH2 is typically stored at -20°C for long-term stability, and it is recommended to avoid freeze-thaw cycles to maintain its activity . When stored properly, the enzyme remains stable and retains its functional properties for extended periods .