BDH1 Human

3-Hydroxybutyrate Dehydrogenase, Type 1 Human Recombinant
Cat. No.
BT7793
Source
E.coli.
Synonyms
D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase, BDH1, SDR9C1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BDH1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (47-343) and having a molecular mass of 35.3kDa.
BDH1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BDH1, a member of the short-chain dehydrogenase/reductase gene family, is a mitochondrial membrane enzyme. It functions as a homotetramer and requires lipids, particularly phosphatidylcholine, for optimal activity. BDH1 plays a crucial role in fatty acid catabolism by catalyzing the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two primary ketone bodies produced during this process.
Description
Recombinant BDH1 Human protein, expressed in E.coli, is a single, non-glycosylated polypeptide chain. It comprises 318 amino acids (residues 47-343) and has a molecular weight of 35.3kDa. The protein includes a 21 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The BDH1 solution is supplied at a concentration of 0.25mg/ml and contains the following components: 20mM Tris-HCl buffer (pH 8.0), 30% glycerol, 0.2M NaCl, 2mM DTT, and 0.1mM PMSF.
Stability
For short-term storage (2-4 weeks), the BDH1 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of BDH1 is greater than 85% as assessed by SDS-PAGE analysis.
Synonyms
D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase, BDH1, SDR9C1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVC SSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPAR SPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDT SPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR.

Product Science Overview

Introduction

3-Hydroxybutyrate Dehydrogenase, Type 1 (BDH1), is an enzyme encoded by the BDH1 gene. This enzyme plays a crucial role in the metabolism of ketone bodies, which are produced during the breakdown of fatty acids. BDH1 is a member of the short-chain dehydrogenase/reductase (SDR) family and is primarily located in the mitochondrial membrane .

Structure and Function

BDH1 forms a homotetrameric lipid-requiring enzyme that is embedded in the mitochondrial membrane . It has a specific requirement for phosphatidylcholine to achieve optimal enzymatic activity . The primary function of BDH1 is to catalyze the interconversion of acetoacetate and ®-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism .

Genetic Information

The BDH1 gene is located on chromosome 3q29 in humans . It encodes a protein that is involved in various metabolic pathways, including ketone body metabolism and general metabolism . The gene has several aliases, including SDR9C1 and D-beta-hydroxybutyrate dehydrogenase .

Clinical Significance

Mutations or dysregulation of the BDH1 gene can be associated with metabolic disorders. For instance, disorders of ketone body transport and chromosome 2Q31.1 duplication syndrome have been linked to BDH1 . The enzyme’s role in ketone body metabolism makes it a potential target for therapeutic interventions in metabolic diseases.

Recombinant BDH1

Recombinant BDH1 refers to the enzyme produced through recombinant DNA technology, which allows for the expression of the human BDH1 gene in various host systems. This recombinant form is used in research to study the enzyme’s function, structure, and potential therapeutic applications.

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