BCL2L11 Human

BCL2 Like 11 Human Recombinant
Cat. No.
BT24554
Source
E.coli.
Synonyms
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM, BAM, BOD.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BCL2L11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 162 amino acids (1-138 a.a) and having a molecular mass of 18.5kDa.
BCL2L11 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Bcl-2-like protein 11 (BCL2L11), a member of the Bcl-2 family, plays a crucial role in apoptosis (programmed cell death). It features a Bcl-2 homology domain 3 (BH3) and is activated by stimuli such as nerve growth factor (NGF) and the transcription factor FKHR-L1. This suggests its involvement in the regulation of apoptosis in neurons and lymphocytes. BCL2L11 interacts with other BCL-2 family members like BCL2, BCL2L1/BCL-X(L), and MCL1, primarily functioning as an activator of apoptosis. The protein exists in several isoforms, including BimEL, BimL, BimS, and Bim-gamma, each with different expression patterns across tissues.
Description
This product consists of a recombinant human BCL2L11 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 162 amino acids (with the BCL2L11 sequence spanning amino acids 1-138) and has a molecular weight of 18.5 kDa. A 24 amino acid His-tag is fused to the N-terminus of the protein to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear and colorless solution that has been sterilized by filtration.
Formulation
The BCL2L11 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at pH 8.0 and contains additional components including 2M Urea, 20% glycerol, 5mM DTT, and 300mM NaCl.
Stability
For optimal storage and to preserve protein integrity, the following guidelines are recommended: If using the entire vial within 2-4 weeks, store at 4°C. For extended storage, freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage to further enhance stability. To maintain protein quality, avoid repeated cycles of freezing and thawing.
Purity
The purity of the BCL2L11 protein is determined to be greater than 80% using SDS-PAGE analysis.
Synonyms
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM, BAM, BOD.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAKQPS DVSSECDREG RQLQPAERPP QLRPGAPTSL QTEPQDRSPA PMSCDKSTQT PSPPCQAFNH YLSAMASMRQ AEPADMRPEI WIAQELRRIG DEFNAYYARR VFLNNYQAAE DHPRMVILRL LRYIVRLVWR MH.

Product Science Overview

Gene and Protein Structure

The BCL2L11 gene is located on chromosome 2 in humans and encodes a protein that contains a Bcl-2 homology domain 3 (BH3). This domain is critical for its interaction with other Bcl-2 family members. The protein exists in multiple isoforms, including BimEL, BimL, and BimS, which differ in their apoptotic potency .

Function and Mechanism

BCL2L11 functions as an apoptotic activator. It interacts with other members of the Bcl-2 family to either promote or inhibit apoptosis. The expression of BCL2L11 can be induced by various factors, including nerve growth factor (NGF) and the forkhead transcription factor FKHR-L1. This suggests that BCL2L11 plays a role in neuronal and lymphocyte apoptosis .

Biological Significance

The protein encoded by BCL2L11 is involved in several critical cellular processes:

  • Apoptosis: BCL2L11 is a key initiator of apoptosis, particularly in thymocyte-negative selection, which is essential for the development of the immune system .
  • Anoikis: This is a form of apoptosis induced when cells detach from the surrounding extracellular matrix, and BCL2L11 is known to induce this process .
  • Disease Association: Dysregulation of BCL2L11 has been linked to various diseases, including Buruli ulcer and Interleukin-7 receptor alpha deficiency .
Research and Applications

Human recombinant BCL2L11 is used in research to study its role in apoptosis and related pathways. It is particularly valuable in understanding the mechanisms of cell death in various diseases and developing potential therapeutic strategies.

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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