BCL2L10 Human

BCL2 Like 10 Human Recombinant
Cat. No.
BT24481
Source
E.coli.
Synonyms
Bcl-2-like protein 10, Bcl2-L-10, Anti-apoptotic protein NrH, Apoptosis regulator Bcl-B, BCL2L10, BCLB, Boo, Diva, BCL-B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BCL2L10 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids (1-172 a.a.) and having a molecular mass of 21.8kDa.
BCL2L10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
BCL2 Like 10 (BCL2L10), a member of the BCL-2 protein family, plays a crucial role in regulating apoptosis. This family is known for forming hetero- or homodimers that function as either anti-apoptotic or pro-apoptotic regulators, impacting various cellular processes. BCL2L10 possesses the conserved BH4, BH1, and BH2 domains, enabling interactions with fellow BCL2 family members such as BCL2, BCL2L1/BCL-X(L), and BAX. Studies show that BCL2L10 overexpression can suppress apoptosis, potentially by preventing cytochrome C release from mitochondria, thus inhibiting caspase-3 activation.
Description
This product consists of a single, non-glycosylated polypeptide chain of BCL2L10 Human Recombinant, produced in E.Coli. It encompasses 195 amino acids (specifically, amino acids 1-172) and exhibits a molecular weight of 21.8kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus, and proprietary chromatographic techniques are employed.
Physical Appearance
The product appears as a clear and colorless solution that has been sterilized by filtration.
Formulation
The BCL2L10 protein solution is provided at a concentration of 0.5mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For optimal stability, store the product at 4°C if it will be used within 2-4 weeks. For longer storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of this product exceeds 90%, as determined by SDS-PAGE analysis.
Synonyms
Bcl-2-like protein 10, Bcl2-L-10, Anti-apoptotic protein NrH, Apoptosis regulator Bcl-B, BCL2L10, BCLB, Boo, Diva, BCL-B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVDQLRE RTTMADPLRE RTELLLADYL GYCAREPGTP EPAPSTPEAA VLRSAAARLR QIHRSFFSAY LGYPGNRFEL VALMADSVLS DSPGPTWGRV VTLVTFAGTL LERGPLVTAR WKKWGFQPRL KEQEGDVARD CQRLVALLSS RLMGQHRAWL QAQGGWDGFC HFFRT.

Product Science Overview

Structure and Function

BCL2L10 contains several conserved domains, including the BH4, BH1, and BH2 domains . These domains are critical for its function and interactions with other proteins in the BCL-2 family. BCL2L10 can form hetero- or homodimers with other BCL-2 family members, influencing their pro- or anti-apoptotic activities .

The primary function of BCL2L10 is to suppress apoptosis. It achieves this by preventing the release of cytochrome c from the mitochondria, a key step in the activation of caspase-3, an enzyme that plays a central role in the execution phase of cell apoptosis . By inhibiting this pathway, BCL2L10 helps in promoting cell survival.

Expression and Localization

BCL2L10 is expressed in various tissues and has been implicated in several physiological and pathological processes. Its expression levels and activity can be altered in different diseases, including cancers and neurodegenerative disorders .

Recombinant BCL2L10

Recombinant BCL2L10 is produced using recombinant DNA technology, where the gene encoding BCL2L10 is cloned and expressed in a suitable host, such as E. coli. The recombinant protein is then purified using chromatographic techniques . The recombinant human BCL2L10 typically consists of 195 amino acids and has a molecular mass of approximately 21.8 kDa .

Applications

Recombinant BCL2L10 is used in various research applications, including:

  • Studying Apoptosis: Researchers use recombinant BCL2L10 to study its role in apoptosis and its interactions with other BCL-2 family members.
  • Drug Development: It serves as a target for developing drugs that can modulate apoptosis, which is crucial in treating diseases like cancer.
  • Biochemical Assays: Recombinant BCL2L10 is used in biochemical assays to understand its binding properties and functional interactions.

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THE BIOTEK
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