BAFF Human, Plant

BAFF (BLyS) Human Recombinant, Plant
Cat. No.
BT13341
Source
Nicotiana benthamiana plant
Synonyms
BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

BAFF human Recombinant produced in Nicotiana benthamiana plant is a single glycosilated polypeptide chain containing 151 amino acids fragment (134-285).
BAFF (C830H1277N223O242S5) is fused to a 10-His-tag at the N-terminal having the total molecular mass of 18-20kDa and purified by standard chromatographic techniques.

Product Specs

Introduction
BAFF, also known as B-cell activating factor, plays a crucial role in the immune system's regulation of B cell activity. It interacts with receptors like TACI and BCMA, similar to APRIL, to stimulate B and T cell functions and regulate humoral immunity. A third receptor, BAFFR, specifically promotes the survival and response of mature B cells. This protein acts as a potent B-cell growth factor in costimulation assays. In mice, administering BAFF can lead to changes in the spleen's B and T cell zones and increased immunoglobulin levels in the blood.
Description
This product consists of a single glycosylated polypeptide chain derived from the BAFF protein found in humans. It's produced using Nicotiana benthamiana plants and encompasses amino acids 134 to 285, totaling 151 amino acids. A 10-His-tag is fused to the N-terminal, resulting in a total molecular mass of 18-20kDa. Purification is achieved using standard chromatographic techniques. The chemical formula of BAFF is C830H1277N223O242S5.
Physical Appearance
The product appears as a sterile, white powder that has been freeze-dried.
Formulation
The lyophilization process is carried out from a solution containing 1mg/ml of BAFF in a 20 mM PBS buffer with a pH of 7 and 0.2 M NaCl.
Solubility
To reconstitute the lyophilized BAFF, it's recommended to dissolve it in sterile 18M-cm H2O at a concentration not lower than 100 µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
The lyophilized BAFF remains stable at room temperature for up to three weeks; however, for extended storage, it's recommended to store it desiccated at a temperature below -18°C. After reconstitution, BAFF can be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is advised. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. It's important to avoid repeated freeze-thaw cycles.
Purity
The purity of the product is determined using SDS-PAGE analysis and is greater than 97.0%.
Biological Activity
The biological activity of the product is evaluated by measuring its ability to stimulate the proliferation of B cells extracted from human PBMCs. This is achieved through a dose-dependent stimulation approach. Cell proliferation is quantified using the MTT method. Please note that the activity results might show variations based on the PBMC donors. The ED50 is typically around 50ng/ml.
Synonyms
BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.
Source
Nicotiana benthamiana plant
Amino Acid Sequence
HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL

Product Science Overview

Introduction

B-cell Activating Factor (BAFF), also known as B Lymphocyte Stimulator (BLyS), is a crucial cytokine in the tumor necrosis factor (TNF) ligand family. It plays a significant role in the survival, proliferation, and differentiation of B cells. BAFF is encoded by the TNFSF13B gene and is also referred to by several other names, including CD257, TALL-1, and THANK .

Structure and Function

BAFF is a 285-amino acid long peptide glycoprotein that undergoes glycosylation at residue 124 . It is expressed by various cell types, including monocytes, macrophages, dendritic cells, and B cells. The cytokine binds to three receptors: TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFF-R) . These interactions are critical for B cell activation and function.

Role in Immune System

BAFF is essential for the maintenance of B cell homeostasis and the regulation of immune responses. It induces B cell proliferation and immunoglobulin secretion, acting as a survival factor for immature, naive, and activated B cells . Elevated levels of BAFF are associated with autoimmune diseases and various hematological malignancies .

Recombinant BAFF Production

Recombinant BAFF (Human) can be produced in various systems, including plants. Plant-based expression systems offer several advantages, such as cost-effectiveness, scalability, and reduced risk of contamination with human pathogens . The recombinant protein retains its biological activity and can be used in research and therapeutic applications.

Therapeutic Applications

BAFF-targeted therapies are being explored for the treatment of autoimmune diseases and B-cell malignancies. Monoclonal antibodies like belimumab, atacicept, and tabalumab have shown promise in clinical trials . These therapies aim to modulate BAFF levels and its interactions with receptors to achieve therapeutic benefits.

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