ATP5F1 Human
Description
ATP5F1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (83-256) and having a molecular mass of 22.6 kDa. ATP5F1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The mitochondrial ATP synthase complex is composed of two main components: the F1 catalytic core and the Fo proton channel. The Fo complex is embedded in the inner mitochondrial membrane and is responsible for proton translocation. The F1 complex protrudes into the mitochondrial matrix and is responsible for ATP synthesis. The ATP5F1 subunit is part of the Fo complex and plays a critical role in the transport of protons across the membrane, which drives the rotation of the F1 complex and the subsequent synthesis of ATP .
The human recombinant version of Synthase Transporting Mitochondrial Fo Complex B1 is produced using recombinant DNA technology. This involves inserting the gene encoding the ATP5F1 protein into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. The recombinant protein is then purified for use in research and therapeutic applications .
The recombinant ATP5F1 protein is widely used in biochemical and biophysical studies to understand the mechanisms of ATP synthesis and mitochondrial function. It is also used in drug discovery and development, particularly in the search for compounds that can modulate mitochondrial function and treat mitochondrial diseases. Additionally, the protein is used in the study of various metabolic disorders and neurodegenerative diseases, where mitochondrial dysfunction is a key factor .
