ATP1B3 Human

ATPaseTransporting Beta 3 Human Recombinant

Cat. No.

BT22181

Source

Escherichia Coli.

Synonyms

ATPase Na+/K+ Transporting Beta 3 Polypeptide, Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic), Sodium/Potassium-Transporting ATPase Subunit Beta-3, Sodium/Potassium-Dependent ATPase Subunit Beta-3, Sodium Pump Subunit Beta-3, ATPB-3, Sodium/Potassium-Transporting ATPase Beta-3 Chain, Sodium/Potassium-Dependent ATPase Beta-3 Subunit, Na K-ATPase Beta-3 Polypeptide, CD298 Antigen, CD298, ATP1B3.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

ATP1B3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (57-279) and having a molecular mass of 27.4 kDa.
ATP1B3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

ATPase Transporting Beta 3 (ATP1B3), a member of the X(+)/potassium ATPases subunit beta family, functions as the non-catalytic component of the active enzyme. This enzyme plays a crucial role in catalyzing the hydrolysis of ATP, which is coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit is responsible for controlling the quantity of sodium pumps transported to the plasma membrane through the assembly of alpha/beta heterodimers. However, the specific role of the beta-3 subunit remains to be fully elucidated.

Description

Recombinantly produced in E.coli, ATP1B3 Human is a single, non-glycosylated polypeptide chain comprising 246 amino acids (57-279) with a molecular mass of 27.4 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.

Physical Appearance

The solution is colorless, sterile, and has been filtered.

Formulation

The ATP1B3 solution is provided at a concentration of 0.25mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.15M NaCl, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to minimize repeated freeze-thaw cycles to maintain product integrity.

Purity

The purity of ATP1B3 Human is determined to be greater than 80.0% based on SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA.

Product Science Overview

Introduction

ATPase Transporting Beta 3, also known as ATP1B3, is a protein that plays a crucial role in the function of the sodium/potassium-transporting ATPase enzyme. This enzyme is essential for maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) ions across the plasma membrane, which are vital for various cellular processes, including osmoregulation, sodium-coupled transport, and electrical excitability of nerve and muscle cells.

Structure and Function

ATP1B3 is a non-catalytic component of the active enzyme complex, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The enzyme complex is composed of two subunits: a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). ATP1B3 belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins and is specifically a part of the Na+/K+ -ATPases subfamily.

The beta subunit, including ATP1B3, is responsible for the assembly of alpha/beta heterodimers, which control the quantity of sodium pumps transported to the plasma membrane. This regulation is crucial for maintaining the proper function and distribution of the sodium/potassium pumps.

Recombinant Production

ATP1B3 Human Recombinant is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 246 amino acids (57-279) and has a molecular mass of 27.4 kDa. The recombinant protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Biological Significance

The sodium/potassium-transporting ATPase, which includes ATP1B3, is integral to various physiological processes. These include:

Osmoregulation: Maintaining the balance of water and electrolytes within cells.
Sodium-Coupled Transport: Facilitating the transport of organic and inorganic molecules.
Electrical Excitability: Ensuring proper function of nerve and muscle cells by maintaining the electrochemical gradients necessary for action potentials.

Clinical Relevance

Mutations or dysregulation of ATP1B3 and the sodium/potassium-transporting ATPase can lead to various medical conditions, including cardiac and neurological disorders. Understanding the function and regulation of ATP1B3 is essential for developing therapeutic strategies for these conditions.

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ATP1B3 Human

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