ATP1B1 Human, Sf9
Description
ATP1B1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
ATPase Transporting Beta 1, also known as ATP1B1, is a crucial component of the Na+/K+ ATPase enzyme complex. This enzyme is integral to maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) ions across the plasma membrane, which is essential for various cellular processes including osmoregulation, sodium-coupled transport, and electrical excitability of nerve and muscle cells .
The Na+/K+ ATPase enzyme is composed of two main subunits: a large catalytic alpha subunit and a smaller glycoprotein beta subunit. The beta subunit, ATP1B1, plays a non-catalytic role but is essential for the proper functioning of the enzyme. It regulates the assembly of alpha/beta heterodimers and the number of sodium pumps transported to the plasma membrane .
ATP1B1 is responsible for the hydrolysis of ATP, which is coupled with the exchange of Na+ and K+ ions across the plasma membrane. This process is vital for maintaining the resting potential of cells, affecting transport mechanisms, and regulating cellular volume. Additionally, ATP1B1 functions as a signal transducer/integrator to regulate pathways such as MAPK, reactive oxygen species (ROS), and intracellular calcium levels .
The Na+/K+ ATPase, including the ATP1B1 subunit, is crucial for maintaining the electrochemical gradients of Na+ and K+ ions. These gradients are essential for various physiological processes, including osmoregulation, sodium-coupled transport of organic and inorganic molecules, and electrical excitability of nerve and muscle cells. The proper functioning of this enzyme is vital for overall cellular homeostasis .
The recombinant form of ATPase Transporting Beta 1 is produced in Sf9 cells, a type of insect cell line derived from the fall armyworm (Spodoptera frugiperda). This recombinant production allows for the generation of high-quality, functional proteins that can be used for research and therapeutic purposes. The recombinant ATP1B1 produced in Sf9 cells is a single, glycosylated polypeptide chain containing 232 amino acids and has a molecular mass of approximately 26.4 kDa .
Recombinant ATP1B1 has various applications in scientific research, particularly in studies related to cellular transport mechanisms, signal transduction pathways, and the development of therapeutic interventions for diseases associated with Na+/K+ ATPase dysfunction. It is also used in the development of assays and diagnostic tools for studying the enzyme’s activity and regulation .
