ASRGL1 Human
ALP, ALP1, CRASH, ,Beta-aspartyl-peptidase, Isoaspartyl dipeptidase.
Description
ASRGL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
The ASRGL1 protein, comprising 308 amino acids, belongs to the Ntn-hydrolase family. Identified as an autoantigenic protein, ASRGL1 is found in the sperm's mid-piece following obstruction of the male reproductive tract. While primarily abundant in the testis, ASRGL1 expression is also observed in the brain, kidney, and gastrointestinal tissues. Notably, elevated levels of ASRGL1 are detected in ovarian, uterine, and mammary tumors compared to their corresponding normal tissues.
ALP, ALP1, CRASH, ,Beta-aspartyl-peptidase, Isoaspartyl dipeptidase.
Product Science Overview
The recombinant human ASRGL1 protein is typically expressed in Escherichia coli and is available in a full-length form, ranging from amino acids 1 to 308 . It is purified to a high degree, with a purity level exceeding 90%, making it suitable for various applications such as SDS-PAGE and mass spectrometry (MS) .
ASRGL1 exhibits both L-asparaginase and beta-aspartyl peptidase activities . The L-asparaginase activity involves the hydrolysis of L-asparagine to L-aspartate and ammonia, which is crucial for the metabolism of asparagine. The beta-aspartyl peptidase activity, on the other hand, involves the cleavage of beta-aspartyl dipeptides and their methyl esters . This dual enzymatic activity is essential for the production of L-aspartate, which can act as an excitatory neurotransmitter in certain brain regions .
ASRGL1 is involved in several biological processes, including the production of L-aspartate, which is important for neurotransmission . It is highly active with substrates such as L-Asp beta-methyl ester and has catalytic activity towards various beta-aspartyl dipeptides . However, it does not exhibit activity towards aspartylglucosaminidase or glutamine .
