ASFV P72
Escherichia Coli.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant African Swine Fever Virus P72 (40-315 aa) produced in E.coli having a Mw of 33kDa.
The African Swine Fever Virus P72 is fused to a 6xHis tag and purified by proprietary chromatographic technique.
Product Specs
Escherichia Coli.
Product Science Overview
African Swine Fever Virus (ASFV) is a highly contagious and lethal virus that affects domestic pigs and wild boars, causing significant economic losses in the swine industry. ASFV is the only member of the Asfarviridae family and is known for its complex structure and large double-stranded DNA genome. The virus is composed of an outer envelope, capsid, inner membrane, core shell, and genome .
The P72 protein, encoded by the B646L gene, is one of the major structural proteins of ASFV. It plays a crucial role in the virus’s ability to infect and replicate within host cells. P72 is highly immunogenic and is a dominant antigen in ASF vaccine and diagnostic research . The protein is involved in the formation of the virus’s icosahedral capsid, which is essential for the virus’s stability and infectivity .
Recombinant P72 protein can be produced using various expression systems. One common method involves cloning the B646L gene into a baculovirus transfer vector, such as pFastBac HTA, to construct recombinant baculoviruses. These recombinant baculoviruses are then used to infect insect cells, which express the P72 protein. The protein is subsequently purified using affinity chromatography techniques .
The P72 protein contains several linear B cell epitopes, which are regions of the protein recognized by the immune system. These epitopes are crucial for the development of diagnostic tools and vaccines. Recent studies have identified seven linear B cell epitopes within the P72 protein, some of which are novel and highly conserved among different ASFV strains . These epitopes can be mapped using overlapping peptides expressed in Escherichia coli and analyzed for their reactivity with monoclonal antibodies .
