ARPC5 Human

Actin Related Protein 2/3 Complex, Subunit 5 Human Recombinant
Cat. No.
BT12479
Source
Escherichia Coli.
Synonyms
Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5, ARC16, MGC88523, dJ127C7.3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARPC5 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 18.4kDa.
The ARPC5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
ARPC5, a 151 amino acid subunit of the Arp2/3 complex, is thought to play a role in maintaining the complex's integrity. Phosphorylation of ARPC5 by MAPKAPK-2 may contribute to Arp2/3 regulatory functions and Actin cytoskeleton remodeling.
Description
Recombinant human ARPC5, expressed in E. coli, is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-151 a.a. of ARPC5 plus a 20 amino acid N-terminal His tag). It has a molecular mass of 18.4 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The ARPC5 solution is provided at a concentration of 0.5 mg/ml in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the ARPC5 protein is greater than 90% as determined by SDS-PAGE.
Synonyms
Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5, ARC16, MGC88523, dJ127C7.3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK NGVDLLMKYI YKGFESPSDN SSAMLLQWHE KALAAGGVGS IVRVLTARKT V.

Product Science Overview

Introduction

Actin Related Protein 2/3 Complex, Subunit 5 (ARPC5) is a crucial component of the actin-related protein 2/3 (Arp2/3) complex. This complex is essential for the regulation of actin polymerization in cells, a process that is fundamental for various cellular functions, including cell motility, vesicle trafficking, and receptor endocytosis . The ARPC5 gene encodes one of the seven subunits of the Arp2/3 complex, specifically the p16 subunit .

Structure and Function

The Arp2/3 complex consists of seven subunits: ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4, and ARPC5 . ARPC5, also known as the p16 subunit, plays a pivotal role in the formation of branched actin networks in the cytoplasm, which provide the force necessary for cell motility . Additionally, the Arp2/3 complex promotes actin polymerization in the nucleus, thereby regulating gene transcription and the repair of damaged DNA .

Evolutionary Conservation

The Arp2/3 complex has been conserved through evolution, indicating its fundamental role in cellular processes . The ARPC5 gene is highly conserved across different species, highlighting its importance in maintaining cellular integrity and function .

Clinical Significance

Mutations in the ARPC5 gene have been associated with various diseases, including Immunodeficiency 113 with Autoimmunity and Autoinflammation, and Leber Hereditary Optic Neuropathy with Demyelinating Disease of the Central Nervous System . These associations underscore the critical role of ARPC5 in maintaining normal cellular functions and its potential impact on human health.

Research and Applications

The study of ARPC5 and the Arp2/3 complex has significant implications for understanding the mechanisms of cell motility and the development of therapeutic strategies for diseases associated with actin polymerization defects . Recombinant human ARPC5 is used in research to study its function and interactions within the Arp2/3 complex, providing insights into its role in cellular processes and disease mechanisms .

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Source
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