ARPC2 Human

Actin Related Protein 2/3 Complex, Subunit 2 Human Recombinant
Cat. No.
BT12325
Source
E.coli.
Synonyms
ARC34, p34-Arc, PNAS-139, PRO2446, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subuni, ARPC2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARPC2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (1-300a.a) and having a molecular mass of 36.7kDa. ARPC2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Actin-related protein 2/3 complex subunit 2 (ARPC2) belongs to the Rho family of small GTPases. It is one of the seven subunits that constitute the human Arp2/3 protein complex. This complex plays a crucial role in regulating actin polymerization within cells and has been conserved throughout evolution. However, the precise function of the ARPC2 subunit (p34 subunit) remains to be fully elucidated.
Description
Recombinant human ARPC2 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 323 amino acids (residues 1-300) and possessing a molecular weight of 36.7 kDa. The ARPC2 protein is fused to a 23-amino acid His-tag at its N-terminus and undergoes purification using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
ARPC2 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 50% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the ARPC2 protein is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
ARC34, p34-Arc, PNAS-139, PRO2446, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subuni, ARPC2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMILLEVN NRIIEETLAL KFENAAAGNK PEAVEVTFAD FDGVLYHISN PNGDKTKVMV SISLKFYKEL QAHGADELLK RVYGSFLVNP ESGYNVSLLY DLENLPASKD SIVHQAGMLK RNCFASVFEK YFQFQEEGKE GENRAVIHYR DDETMYVESK KDRVTVVFST VFKDDDDVVI GKVFMQEFKE GRRASHTAPQ VLFSHREPPL ELKDTDAAVG DNIGYITFVL FPRHTNASAR DNTINLIHTF RDYLHYHIKC SKAYIHTRMR AKTSDFLKVL NRARPDAEKK EMKTITGKTF SSR.

Product Science Overview

Introduction

The Actin Related Protein 2/3 Complex, Subunit 2 (ARPC2) is a crucial component of the Arp2/3 complex, a multiprotein assembly that plays a significant role in the regulation of the actin cytoskeleton. This complex is essential for various cellular processes, including cell motility, shape, and intracellular transport.

Structure and Composition

The Arp2/3 complex consists of seven subunits: Arp2, Arp3, ARPC1A, ARPC1B, ARPC2, ARPC3, ARPC4, and ARPC5 . ARPC2, also known as p34-Arc, is one of these subunits and is integral to the complex’s function. The ARPC2 subunit is approximately 34 kDa in size and is encoded by the ARPC2 gene located on chromosome 2 in humans .

Function

The primary function of the Arp2/3 complex is to initiate the formation of branched actin networks. This is achieved through the nucleation of new actin filaments, a process that is stimulated by nucleation-promoting factors (NPFs) . The complex binds to the sides of existing actin filaments and creates a new branch, thereby generating a dense and dynamic actin network.

ARPC2, as part of the Arp2/3 complex, contributes to various cellular activities:

  • Actin Polymerization: It facilitates the polymerization of actin filaments, which is crucial for cell movement and stability .
  • Cell Motility: The complex provides the force required for cell motility by forming branched actin networks in the cytoplasm .
  • Nuclear Functions: In addition to its role in the cytoplasm, the Arp2/3 complex also promotes actin polymerization in the nucleus, which is important for gene transcription and DNA repair .
Biological Significance

The Arp2/3 complex, including ARPC2, is vital for maintaining the structural integrity of the cytoskeleton. It is involved in various cellular processes such as:

  • Phagocytosis: Regulation of actin dynamics for the formation of phagocytic cups .
  • Endocytosis: Clathrin-mediated endocytosis, which is essential for the internalization of various molecules .
  • Cell Spreading: Positive regulation of substrate adhesion-dependent cell spreading .
Clinical Relevance

Mutations or dysregulation of the ARPC2 gene can lead to various diseases. For instance, abnormalities in the Arp2/3 complex have been associated with endometrial type cervical adenomyoma and cervical adenomyoma . Understanding the function and regulation of ARPC2 is crucial for developing therapeutic strategies for these conditions.

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Actin Related Protein 2/3 Complex, Subunit 5 Human Recombinant
Cat. No.
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Source
Escherichia Coli.
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