ARL2 Human

ADP-Ribosylation Factor-Like 2 Human Recombinant
Cat. No.
BT13930
Source
Escherichia Coli.
Synonyms
ADP-ribosylation factor-like protein 2, ARL2, ARFL2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARL2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids (1-184 a.a.) and having a molecular mass of 23kDa. The ARL2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
ARL2, a member of the ADP-ribosylation factor (ARF) family, participates in a secretory pathway responsible for the calcium-dependent release of acetylcholine. It plays a crucial role in tubule protein folding, impacting microtubule dynamics and cell cycle progression. Unlike other ARF members, ARL2 does not activate cholera toxin's catalytic subunit.
Description
This product consists of a single, non-glycosylated polypeptide chain of human ARL2, recombinantly produced in E. coli. It includes a 20 amino acid His tag at the N-terminus and encompasses amino acids 1-184 of the ARL2 sequence. With a molecular weight of 23kDa, the protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ARL2 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1mM PMSF, 1mM DTT, and 40% Glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90%, as determined by SDS-PAGE analysis.
Synonyms
ADP-ribosylation factor-like protein 2, ARL2, ARFL2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGLLTILKKM KQKERELRLL MLGLDNAGKT TILKKFNGED IDTISPTLGF NIKTLEHRGF KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ DCQRELQSLL VEERLAGATL LIFANKQDLP GALSSNAIRE ALELDSIRSH HWCIQGCSAV TGENLLPGID WLLDDISSRI FTAD.

Product Science Overview

Structure and Function

ARL2 is a small GTPase that cycles between an inactive GDP-bound form and an active GTP-bound form. This cycling is essential for its function as a molecular switch, regulating various cellular activities. Unlike other ARF proteins, ARL2 does not associate with membranes upon activation, suggesting distinct functional roles .

Interaction and Regulation

ARL2 interacts specifically with ARL2-binding protein (ARL2BP), which is considered its first specific effector. This interaction is crucial for ARL2’s function, as ARL2BP does not interact with other ARF proteins or RHO proteins . The binding of ARL2 to GTP is characterized by high affinity, and this interaction is essential for its regulatory roles.

Biological Significance

ARL2 plays a significant role in the regulation of microtubule dynamics and mitochondrial function. It is involved in the transport of proteins to the mitochondria and the regulation of mitochondrial morphology. Additionally, ARL2 has been implicated in the regulation of cell division and the maintenance of cellular homeostasis .

Recombinant ARL2

The recombinant form of ARL2 is produced using an expression system, typically in E. coli, to ensure high purity and activity. This recombinant protein is used in various research applications, including studies on protein-protein interactions, cellular signaling pathways, and the functional analysis of ARL2 in different biological contexts .

Research Applications

Recombinant ARL2 is widely used in biochemical and cell biology research to study its role in cellular processes. It is also used in structural biology to understand the conformational changes associated with its activation and interaction with other proteins. The availability of recombinant ARL2 has facilitated the development of assays to investigate its function and regulation in various cellular contexts .

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