ARL5A Human

ARL5A, also known as ADP-ribosylation factor-like protein 5A, is a protein encoded by the ARL5A gene in humans. Unlike many members of the ARF family, ARL5A is thought to lack ADP-ribosylation enhancing activity. This protein is developmentally regulated and may play a role in nuclear dynamics and signaling cascades during embryonic development .

Recombinant human ARL5A protein is typically produced using Escherichia coli (E. coli) expression systems. The protein is fused to a His-tag at the N-terminus to facilitate purification. The recombinant protein is then purified using conventional chromatography techniques to achieve a purity of over 90% .

ARL5A is involved in various cellular processes, including vesicular trafficking and phospholipase D activation. The protein’s GTP-binding activity is essential for its function. The recombinant human ARL5A protein has been used in various research applications, including SDS-PAGE and mass spectrometry (MS), to study its structure and function .

ARL5A is important in eukaryotic vesicular trafficking pathways, which are crucial for the transport of molecules within cells. The protein’s role in nuclear dynamics and signaling cascades during embryonic development highlights its significance in developmental biology .

Recombinant human ARL5A protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s stability and activity. The protein is typically stored in a buffer containing 20 mM Tris-HCl (pH 8.0), 2 mM dithiothreitol (DTT), 20% glycerol, and 0.2 mM phenylmethylsulfonyl fluoride (PMSF) .

Recombinant human ARL5A protein is used in various research applications, including:

• SDS-PAGE: To analyze the protein’s purity and molecular weight.
• Mass Spectrometry (MS): To study the protein’s structure and post-translational modifications.
• Cytometric Bead Assay: To investigate the protein’s biological activity .