ARHGDIA Human

Rho GDP dissociation inhibitor (GDI) alpha Human Recombinant
Cat. No.
BT15496
Source
Escherichia Coli.
Synonyms
Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1, RHOGDI, RHOGDI-1, MGC117248.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ARHGDIA Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids (24-204 a.a.) and having a molecular mass of 22.9kDa. The ARHGDIA is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Rho GDP-dissociation inhibitor 1 (ARHGDIA), a member of the RAS gene superfamily, functions as a small guanine nucleotide exchange (GTP/GDP) factor. It primarily resides in the cytoplasm and inhibits GDP dissociation from Rho proteins. This inhibition prevents GTP binding and subsequent activation of Rho proteins. In humans, ARHGDIA can be phosphorylated at Ser 101 by p21-activated kinase, which inhibits its activity and potentially leads to positive feedback regulation of its target proteins.
Description
Recombinant human ARHGDIA, expressed in E. coli, is a non-glycosylated polypeptide chain with a 21 amino acid His tag at its N-terminus. It encompasses amino acids 24-204, resulting in a molecular weight of 22.9 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The ARHGDIA solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Multiple freeze-thaw cycles should be avoided.
Purity
The purity of the ARHGDIA is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1, RHOGDI, RHOGDI-1, MGC117248.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSVNYKPPAQ KSIQEIQELD KDDESLRKYK EALLGRVAVS ADPNVPNVVV TGLTLVCSSA PGPLELDLTG DLESFKKQSF VLKEGVEYRI KISFRVNREI VSGMKYIQHT YRKGVKIDKT DYMVGSYGPR AEEYEFLTPV EEAPKGMLAR GSYSIKSRFT DDDKTDHLSW EWNLTIKKDW KD.

Product Science Overview

Introduction

Rho GDP Dissociation Inhibitor (GDI) Alpha, also known as ARHGDIA, is a crucial protein involved in the regulation of Rho GTPases. These GTPases are part of the Ras superfamily and play significant roles in various cellular processes, including cell motility, adhesion, proliferation, and survival .

Gene and Protein Structure

The ARHGDIA gene encodes the Rho-GDI alpha protein, which is responsible for maintaining Rho GTPases in their inactive state by inhibiting the dissociation of GDP (guanine diphosphate) from these molecules . This inhibition is essential for controlling the activity of Rho proteins such as CDC42, RAC1, and RHOA .

Mechanism of Action

Rho-GDI alpha functions by binding to the GDP-bound form of Rho GTPases, preventing their activation. This binding retains the Rho proteins in an inactive cytosolic pool, regulating their stability and protecting them from degradation . Additionally, Rho-GDI alpha is actively involved in the recycling and distribution of activated Rho GTPases within the cell .

Biological Significance

The activity of Rho-GDI alpha is vital for various cellular processes. It regulates the actin cytoskeleton, which is crucial for cell shape, motility, and division . Moreover, the protein’s role in maintaining Rho GTPases in an inactive state is essential for proper cellular signaling and function .

Clinical Relevance

Alterations in the expression of ARHGDIA have been associated with several diseases, including nephrotic syndrome type 8 and genetic steroid-resistant nephrotic syndrome . Furthermore, changes in Rho-GDI alpha expression have been observed in various cancers, where it is linked to the malignant phenotype, including increased migration, invasion, metastasis, and resistance to anticancer agents .

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Source
E.coli.
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