AGO2 (1-200) Human
Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, AGO2, EIF2C2, Protein slicer.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
AGO2 (1-200) Human Recombinant is a single, non-glycosylated, polypeptide chain containing 210 amino acids (1-200 a.a) and having a molecular mass of 23.7kDa (calculated). AGO2 (1-200) is fused to a 10 a.a His tag at N-terminal.
Product Specs
The Argonaute protein plays a crucial role in RNA silencing as a core component of the RNA-induced silencing complex (RISC). This complex is responsible for RNA interference (RNAi). Argonaute proteins bind to small non-coding RNA fragments through base pairing, ultimately leading to the degradation of messenger RNA or the suppression of translation.
This product consists of a single, non-glycosylated polypeptide chain comprising amino acids 1-200 of the human AGO2 protein. It has a molecular weight of 23.7kDa (calculated) and is not glycosylated. A 10 amino acid His tag is fused to the N-terminus of the protein.
This product is supplied as a 0.5mg/ml solution in 50mM acetate buffer, pH 4. It is sterile filtered (0.4 µm) and lyophilized.
To create a working solution of approximately 0.5mg/ml, it is advised to reconstitute the lyophilized powder in 0.1M acetate buffer (pH 4) and allow it to fully dissolve. Please note that this product is not sterile. Before using in cell culture, it is essential to filter the product through a sterile filter of appropriate pore size.
The purity is determined to be greater than 90% via SDS-PAGE analysis.
Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, AGO2, EIF2C2, Protein slicer.
MKHHHHHHAS MYSGAGPALA PPAPPPPIQG YAFKPPPRPD FGTSGRTIKL QANFFEMDIP KIDIYHYELD IKPEKCPRRV NREIVEHMVQ HFKTQIFGDR KPVFDGRKNL YTAMPLPIGR DKVELEVTLP GEGKDRIFKV SIKWVSCVSL QALHDALSGR LPSVPFETIQ ALDVVMRHLP SMRYTPVGRS FFTASEGCSN PLGGGREVW.
Product Science Overview
The recombinant Argonaute 2 (1-200 a.a.) is a single, non-glycosylated polypeptide chain consisting of 210 amino acids, including a 10 amino acid His tag at the N-terminal . This segment has a calculated molecular mass of approximately 23.7 kDa . The protein is typically expressed in Escherichia coli and is provided as a lyophilized powder for research use .
Argonaute 2 is a key component of the RNA-induced silencing complex (RISC), which mediates the RNAi process . In this pathway, small non-coding RNA fragments, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), guide the RISC to target messenger RNAs (mRNAs) for cleavage or translational suppression . AGO2 is unique among the Argonaute family members because it possesses endonuclease activity, allowing it to directly cleave target mRNAs .
The recombinant form of Argonaute 2 (1-200 a.a.) is used extensively in molecular biology and genetic research. It serves as a tool to study the mechanisms of RNAi and gene regulation. Researchers utilize this protein to investigate the interactions between AGO2 and various RNA molecules, as well as to understand its role in gene silencing .
