AGO2 Human
Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, AGO2, EIF2C2, Protein slicer.
Filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
AGO2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 1-859) containing 869 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 98.4kDa (calculated).
Product Specs
Argonaute proteins are key components of the RNA-induced silencing complex (RISC), which plays a crucial role in RNA interference (RNAi). These proteins bind to small non-coding RNA fragments, such as microRNAs (miRNAs) or small interfering RNAs (siRNAs), through base pairing. This interaction guides the RISC to target messenger RNA (mRNA) molecules, leading to either mRNA cleavage or translation suppression, effectively silencing gene expression.
This product consists of a recombinant human AGO2 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 869 amino acids, including a 10-amino acid N-terminal His tag. The calculated molecular mass of the protein is 98.4 kDa.
The product appears as a clear, colorless solution after filtration.
The AGO2 protein is supplied as a filtered solution in a buffer containing 50 mM acetate (pH 4.0) and 20% (w/v) glycerol.
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
The purity of the AGO2 protein is determined to be greater than 90% using SDS-PAGE analysis.
Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, eIF-2C 2, eIF2C 2, PAZ Piwi domain protein, PPD, AGO2, EIF2C2, Protein slicer.
MKHHHHHHAS MYSGAGPALA PPAPPPPIQG YAFKPPPRPD FGTSGRTIKL QANFFEMDIP KIDIYHYELD IKPEKCPRRV NREIVEHMVQ HFKTQIFGDR KPVFDGRKNL YTAMPLPIGR DKVELEVTLP GEGKDRIFKV SIKWVSCVSL QALHDALSGR LPSVPFETIQ ALDVVMRHLP SMRYTPVGRS FFTASEGCSN PLGGGREVWF GFHQSVRPSL WKMMLNIDVS ATAFYKAQPV IEFVCEVLDF KSIEEQQKPL TDSQRVKFTK EIKGLKVEIT HCGQMKRKYR VCNVTRRPAS HQTFPLQQES GQTVECTVAQ YFKDRHKLVL RYPHLPCLQV GQEQKHTYLP LEVCNIVAGQ RCIKKLTDNQ TSTMIRATAR SAPDRQEEIS KLMRSASFNT DPYVREFGIM VKDEMTDVTG RVLQPPSILY GGRNKAIATP VQGVWDMRNK QFHTGIEIKV WAIACFAPQR QCTEVHLKSF TEQLRKISRD AGMPIQGQPC FCKYAQGADS VEPMFRHLKN TYAGLQLVVV ILPGKTPVYA EVKRVGDTVL GMATQCVQMK NVQRTTPQTL SNLCLKINVK LGGVNNILLP QGRPPVFQQP VIFLGADVTH PPAGDGKKPS IAAVVGSMDA HPNRYCATVR VQQHRQEIIQ DLAAMVRELL IQFYKSTRFK PTRIIFYRDG VSEGQFQQVL HHELLAIREA CIKLEKDYQP GITFIVVQKR HHTRLFCTDK NERVGKSGNI PAGTTVDTKI THPTEFDFYL CSHAGIQGTS RPSHYHVLWD DNRFSSDELQ ILTYQLCHTY VRCTRSVSIP APAYYAHLVA FRARYHLVDK EHDSAEGSHT SGQSNGRDHQ ALAKAVQVHQ DTLRTMYFA.
Product Science Overview
Ago2 contains three RNA-binding domains:
- PIWI Domain: This domain is essential for the catalytic activity of Ago2, enabling it to cleave target mRNAs.
- MID Domain: Responsible for anchoring the mono-phosphorylated 5′-end of the guide RNA.
- PAZ Domain: Binds the hydroxylated 3′-end of the guide RNA .
These domains work together to facilitate the binding and processing of microRNAs (miRNAs) and small interfering RNAs (siRNAs), which guide Ago2 to its target mRNAs.
Ago2 is a core component of the RNA-induced silencing complex (RISC). It binds to mature miRNAs and siRNAs, which serve as guides to identify complementary sequences in target mRNAs. Once bound, Ago2 can either cleave the target mRNA or repress its translation, depending on the degree of complementarity between the guide RNA and the target .
miRNAs are short noncoding RNAs, approximately 22 nucleotides in length, that regulate gene expression at the post-transcriptional level. They are involved in various cellular processes, including development, differentiation, and stress responses. It is estimated that more than 50% of human protein-coding genes are regulated by miRNAs .
Ago2’s ability to bind and process miRNAs and siRNAs makes it a pivotal player in gene regulation. It helps maintain cellular homeostasis by fine-tuning the expression of numerous genes.
Recombinant human Ago2 is produced using genetic engineering techniques, where the human Ago2 gene is cloned and expressed in a suitable host system, such as bacteria or mammalian cells. This allows for the production of large quantities of purified Ago2 protein for research purposes. Recombinant Ago2 is used in various studies to understand its structure, function, and role in RNAi and gene regulation .
Understanding the function and mechanisms of Ago2 has significant implications for biomedical research and potential therapeutic applications. For instance, manipulating Ago2 activity could lead to novel treatments for diseases where gene regulation is disrupted, such as cancer, cardiovascular diseases, and autoimmune disorders .
