AREG Human

The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor . Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been identified . Amphiregulin is expressed in various parts of the body, including the ovary, placenta, pancreas, breast, lung, and spleen .

Amphiregulin interacts with the epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells . It plays a crucial role in mammary ductal development and has been implicated in driving human breast cancer progression . Additionally, it drives the proliferation of fibroblasts and the production of cytokines such as interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF), suggesting a proinflammatory role observed in conditions like rheumatoid arthritis .

Recombinant human Amphiregulin is typically produced in E. coli and is supplied in a lyophilized form . It is used in various research applications, including cell proliferation assays. The bioactivity of recombinant human Amphiregulin is determined in a 3T3 cell proliferation assay, with an effective dose (ED50) of less than or equal to 20 ng/mL .

Recombinant human Amphiregulin is used in cell or tissue culture and as an ELISA standard . It is recommended to reconstitute the lyophilized protein in sterile water or PBS, with the addition of a carrier protein like BSA for long-term storage . The stability and storage conditions vary, but it is generally stable for 12 months at -20 to -70°C as supplied, and for 1 to 3 months after reconstitution under sterile conditions .