APRIL Mouse
APRIL Mouse Recombinant
Cat. No.
BT24131
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13, April.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
APRIL Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.4 kDa. The APRIL is purified by proprietary chromatographic techniques.
Product Specs
Introduction
APRIL, a member of the TNF ligand superfamily (TNFSF13), is a type II transmembrane protein. APRIL expression is typically low in tissues but increases in various tumor types and transformed cell lines. APRIL promotes the proliferation of tumor cell lines and enhances tumorigenicity in nude mice.
Description
Recombinant Mouse APRIL, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 146 amino acids with a molecular weight of 16.4 kDa. The APRIL protein undergoes purification using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS), pH 7.4, containing 0.02% Tween-20.
Solubility
To reconstitute the lyophilized APRIL, it is advised to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized APRIL remains stable at room temperature for up to three weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, store APRIL at 4°C for 2-7 days. For long-term storage, freeze APRIL below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0%, as determined by SDS-PAGE analysis.
Biological Activity
Biological activity is assessed through a cell proliferation assay using activated T cells.
Synonyms
Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13, April.
Source
Escherichia Coli.
Amino Acid Sequence
AVLTQKHKKK HSVLHLVPVN ITSKADSDVT EVMWQPVLRR GRGLEAQGDI VRVWDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIRSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL.
Product Science Overview
Structure and Synthesis
Biological Functions
Applications
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