APRIL Human
Description
Product Specs
Product Science Overview
APRIL is synthesized as a 32 kDa proprotein, which is cleaved by the enzyme furin in the Golgi apparatus to release the active 17 kDa soluble molecule . The human APRIL gene codes for at least four alternatively spliced transcriptional variants, resulting in different isoforms of the APRIL precursor protein . These isoforms can be cleaved to release a soluble C-terminal fragment that comprises the TNF-like receptor binding domain of the APRIL precursor .
APRIL is expressed in various cell types, including monocytes, macrophages, certain transformed cell lines, and certain cancers of the colon and lymphoid tissues . It competes with another TNF family member, BAFF, for two receptors: TACI and BCMA . Both APRIL and BAFF stimulate the proliferation of B and T cells . Additionally, APRIL has the ability to stimulate the proliferation of various tumor cell lines, including Jurkat T cells and MCF-7 carcinoma cells .
Recombinant human APRIL is a soluble 16.3 kDa protein consisting of 146 amino acid residues . It is commonly used in research to study its effects on cell proliferation and immune regulation. For example, APRIL has been shown to induce cell death in Jurkat cells and stimulate the proliferation of anti-IgM stimulated mouse B cells . These properties make APRIL a promising molecule for potential applications in immunotherapy .
Recombinant human APRIL is typically expressed in E. coli and purified to ensure high purity and low endotoxin levels . The protein is often presented in a buffer solution and lyophilized for storage . It is important to handle and store the protein under specific conditions to maintain its stability and activity .
