APOM Human
Description
Product Specs
MGSSHHHHHH SSGLVPRGSH MCPEHSQLTT LGVDGKEFPE VHLGQWYFIA GAAPTKEELA TFDPVDNIVF NMAAGSAPMQ LHLRATIRMK DGLCVPRKWI YHLTEGSTDL RTEGRPDMKT ELFSSSCPGG IMLNETGQGY QRFLLYNRSP HPPEKCVEEF KSLTSCLDSK AFLLTPRNQE ACELSNN.
Product Science Overview
Apolipoprotein-M (ApoM) is a unique member of the apolipoprotein family, which plays a crucial role in lipid metabolism and cardiovascular health. ApoM is primarily associated with high-density lipoproteins (HDL) and, to a lesser extent, with low-density lipoproteins (LDL) and triglyceride-rich lipoproteins. This protein is secreted through the plasma membrane but remains membrane-bound, participating in lipid transport .
ApoM is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of the lipocalin family proteins . It functions as a component of lipoprotein particles, which are essential for fatty acid and cholesterol transport and metabolism . The protein is involved in the binding and transport of small lipophilic molecules, including sphingosine-1-phosphate (S1P), which is crucial for vascular development and immune cell trafficking .
Recombinant human ApoM is typically produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 187 amino acids. The recombinant protein is fused to a 21 amino acid His-Tag at the N-terminus and purified by standard chromatography techniques . The protein is often lyophilized from a filtered solution in phosphate-buffered saline (PBS) and can be reconstituted at a concentration of 500 μg/mL in PBS .
ApoM’s ability to bind small lipophilic molecules, such as all-trans-retinoic acid, is measured by its capacity to quench tryptophan fluorescence. The concentration of all-trans-retinoic acid required to quench 50% of tryptophan fluorescence in recombinant human ApoM is approximately 10-50 μM . This binding ability highlights ApoM’s role in lipid metabolism and its potential implications in cardiovascular diseases .
ApoM has gained significant attention in the field of lipid metabolism and cardiovascular health due to its unique properties and functions. Understanding the intricate nature of ApoM sheds light on its significance as a potential biomarker and therapeutic target in cardiovascular disorders . Research into ApoM continues to provide valuable insights into its role in lipid transport and its impact on human health.
