APOB Human
Human Plasma.
APOB, APO-B, Apolipoprotein B.
Sterile Filtered lyophilized (freeze-dried) powder.
Greater than 95.0%.
Description
Human APOB produced from Human plasma having a molecular mass of 550 kDa.
Product Specs
Apolipoprotein B (ApoB) is a vital protein responsible for transporting lipids, including cholesterol, throughout the body. It's the primary protein found in LDL (low-density lipoprotein), IDL (intermediate-density lipoprotein), VLDL (very low-density lipoprotein), and chylomicrons—all particles that carry lipids in the watery environment surrounding cells. While its exact functions within these particles are not fully understood, ApoB is essential for their formation and serves as a docking point for LDL receptors on cells, enabling the uptake of lipids.
This product consists of human ApoB, derived from human plasma, with a molecular weight of 550 kDa.
Sterile Filtered lyophilized (freeze-dried) powder.
This ApoB product has been lyophilized from a solution containing 0.5mg/ml ApoB in a buffer of 50mM Na2CO3 (pH 10), 50mM NaCl, and 10mM sodium deoxycholate.
To reconstitute the lyophilized ApoB, it is recommended to dissolve it in sterile 18M-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Lyophilized ApoB is stable at room temperature for up to 3 weeks. However, for long-term storage, it should be stored desiccated at a temperature below -18°C. After reconstitution, the ApoB solution should be stored at 4°C and is stable for 2-7 days. For extended storage, adding a carrier protein like HSA or BSA to a final concentration of 0.1% is recommended. Avoid repeated freeze-thaw cycles.
Greater than 95.0% pure.
The donor of the starting material has been tested and confirmed negative for antibodies to HIV-1, HIV-2, HCV, and HBSAG.
APOB, APO-B, Apolipoprotein B.
Human Plasma.
Product Science Overview
ApoB exists in two main forms:
- ApoB-100: This is the most abundant form and is synthesized in the liver. It is a primary component of LDL, VLDL, and IDL particles .
- ApoB-48: This form is produced in the intestine and is a crucial component of chylomicrons, which transport dietary lipids from the intestine to other parts of the body .
Elevated levels of ApoB are associated with an increased risk of atherosclerotic cardiovascular disease. ApoB measurement is considered a more precise indicator of cardiovascular risk compared to traditional lipid measurements like LDL cholesterol . High levels of ApoB can lead to the formation of plaques in blood vessels, contributing to conditions such as heart disease and stroke .
Mutations in the APOB gene can lead to various lipid disorders:
- Hypobetalipoproteinemia: Characterized by abnormally low levels of ApoB, leading to malabsorption of dietary fats and fat-soluble vitamins .
- Hyperapobetalipoproteinemia (HALB): A condition with disproportionately high levels of ApoB relative to LDL cholesterol, increasing the risk of coronary heart disease .
