APOE3 Human

Apolipoprotein E3 (Human Recombinant) is a synthetic version of the naturally occurring Apolipoprotein E3, a protein that plays a crucial role in lipid metabolism and brain health. This recombinant protein is produced using advanced biotechnological methods, ensuring high purity and biological activity.

Apolipoprotein E3 is a major component of several lipoproteins, including low-density lipoproteins (LDL), very low-density lipoproteins (VLDL), high-density lipoproteins (HDL), and chylomicrons . It is primarily produced by hepatocytes, macrophages, and non-neuronal cells in the central nervous system . The protein is involved in the transport of triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism .

The recombinant form of Apolipoprotein E3 is typically produced in Escherichia coli or other expression systems such as Baculovirus-mediated expression in Spodoptera frugiperda cells . The production process involves the insertion of the human Apolipoprotein E3 gene into the host cells, which then express the protein. The recombinant protein is purified to achieve high purity levels, often exceeding 90% .

Recombinant Apolipoprotein E3 retains the biological activity of the native protein. It is known to stimulate the proliferation of human SH-SY5Y cells and bind to lipoprotein receptors, facilitating lipid transport and metabolism . The protein’s ability to interact with various lipoprotein receptors makes it a valuable tool for studying lipid metabolism and related diseases.

Apolipoprotein E3 (Human Recombinant) is widely used in research to study lipid metabolism, cardiovascular diseases, and neurodegenerative disorders. Its role in lipid transport and receptor binding makes it a critical component in understanding the mechanisms underlying these conditions. Additionally, it is used in the development of therapeutic strategies for diseases such as Alzheimer’s disease, where lipid metabolism and transport are disrupted .

Recombinant Apolipoprotein E3 should be stored at -20°C to -80°C for long-term storage and at 2°C to 8°C for short-term use . It is recommended to reconstitute the protein in a buffer containing a carrier protein or stabilizer to maintain its stability and biological activity .