APOC1 Human

Apolipoprotein C-I Human Recombinant

Cat. No.

BT11563

Source

Escherichia Coli.

Synonyms

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

APOC1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 80 amino acids (27-83 a.a.) and having a molecular mass of 9.0kDa. APOC1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Apolipoprotein C-I (APOC1), primarily produced in the liver, belongs to the apolipoprotein C family. Commonly present in plasma, APOC1 activates esterified lechitin cholesterol, playing a crucial role in esterified cholesterol exchange among lipoproteins and cholesterol removal from tissues. During monocyte differentiation into macrophages, APOC1 is activated. This protein's primary function is to inhibit CETP by modifying the electric charge of HDL molecules. Furthermore, APOC1 binds free fatty acids, reducing their intracellular esterification.

Description

Recombinant human APOC1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 80 amino acids (27-83 a.a.). It has a molecular mass of 9.0 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and undergoes purification using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The APOC1 protein solution (0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, and 10% glycerol.

Stability

For short-term storage (2-4 weeks), the product should be kept at 4°C. Long-term storage requires freezing at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage durations. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeds 90% as determined by SDS-PAGE analysis.

Synonyms

Apolipoprotein C-I, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSTPDVSSA LDKLKEFGNT LEDKARELIS RIKQSELSAK MREWFSETFQ KVKEKLKIDS.

Product Science Overview

Structure and Genetics

Apolipoprotein C-I is encoded by the APOC1 gene, located on chromosome 19 in humans. The protein consists of 57 amino acids and is a component of various lipoproteins, including very-low-density lipoproteins (VLDL) and high-density lipoproteins (HDL). The gene’s expression is regulated by several factors, including dietary intake and hormonal signals.

Function and Mechanism

ApoC-I is involved in several key processes:

Lipid Transport: It plays a role in the transport of lipids by binding to lipoproteins.
Enzyme Inhibition: ApoC-I inhibits the activity of lipoprotein lipase and hepatic lipase, enzymes that are crucial for the hydrolysis of triglycerides.
Cholesterol Metabolism: It is involved in the exchange of esterified cholesterol between lipoproteins and the removal of cholesterol from tissues.

Clinical Significance

ApoC-I has been implicated in various diseases, particularly those related to lipid metabolism and cardiovascular health. Elevated levels of ApoC-I are associated with hyperlipidemia and an increased risk of atherosclerosis. Additionally, it has been studied in the context of Alzheimer’s disease, where it may influence the deposition of amyloid-beta plaques.

Recombinant ApoC-I

Recombinant ApoC-I is produced using genetic engineering techniques, where the APOC1 gene is inserted into a host organism, typically bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in research to study its function and potential therapeutic applications.

Research and Future Directions

Recent studies have focused on the role of ApoC-I in immune regulation and its potential as a therapeutic target for cardiovascular and neurodegenerative diseases. Understanding the precise mechanisms by which ApoC-I influences lipid metabolism and immune responses could lead to new treatments for these conditions.

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APOC1 Human

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