APOA5 Human, HEK
Filtered White lyophilized (freeze-dried) powder.
Description
Product Specs
The product appears as a white powder that has been freeze-dried and filtered.
Product Science Overview
Apolipoprotein A-V (ApoA-V) is a protein that plays a crucial role in lipid metabolism, particularly in the regulation of plasma triglyceride levels. Elevated triglyceride levels are a significant risk factor for cardiovascular diseases, making ApoA-V an important target for research and therapeutic interventions. The recombinant form of this protein, expressed in Human Embryonic Kidney (HEK) cells, is widely used in scientific studies to understand its function and potential applications.
Apolipoprotein A-V is a 40.1 kDa protein consisting of 354 amino acid residues . The recombinant form is often tagged with a flag tag for ease of purification and detection. The protein is expressed in HEK 293 cells, a human cell line that provides a suitable environment for proper protein folding and post-translational modifications .
ApoA-V is primarily involved in the regulation of plasma triglyceride levels. It acts by enhancing the hydrolysis of triglycerides in very low-density lipoproteins (VLDL) and chylomicrons, facilitating their clearance from the bloodstream. This function is critical in maintaining lipid homeostasis and preventing hypertriglyceridemia, a condition associated with an increased risk of cardiovascular diseases .
Mutations in the APOA5 gene, which encodes ApoA-V, have been linked to various lipid disorders. For instance, certain polymorphisms are associated with hypertriglyceridemia and an increased risk of coronary artery disease. Understanding the role of ApoA-V in lipid metabolism can lead to the development of novel therapeutic strategies for managing dyslipidemia and related cardiovascular conditions .
The recombinant form of ApoA-V is used in various research applications, including:
- Western Blotting (WB): To detect and quantify the protein in different samples.
- Enzyme-Linked Immunosorbent Assay (ELISA): For measuring the protein’s concentration in biological fluids.
- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE): To analyze the protein’s purity and molecular weight .
Recombinant ApoA-V is typically lyophilized and can be reconstituted in deionized water to prepare a working stock solution. It is recommended to store the lyophilized protein at -20°C and avoid repeated freeze-thaw cycles to maintain its stability. Once reconstituted, the protein can be stored at 4°C for a limited period without significant degradation .
