AP1AR Human
Description
AP1AR is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The AP1AR gene is located on chromosome 4q25 and encodes a protein consisting of 302 amino acids . The protein contains a coiled-coil region, which is essential for its interaction with other proteins, such as AP1G1 (gamma1-adaptin), a subunit of the AP-1 adaptor complex . This interaction is critical for the regulation of membrane association and the release of the AP-1 complex from membranes .
AP1AR is necessary for AP-1-dependent transport between the TGN and endosomes . It regulates the membrane association of AP1G1/gamma1-adaptin by directly interacting with it, which attenuates the release of the AP-1 complex from membranes . This regulation is crucial for maintaining proper intracellular trafficking and ensuring that proteins are correctly sorted and transported within the cell .
Additionally, AP1AR is involved in the negative regulation of receptor recycling and vesicle targeting from the trans-Golgi to endosomes . It also plays a role in linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic . This linkage is essential for the proper functioning of intracellular transport mechanisms and the maintenance of cellular homeostasis .
The proper functioning of AP1AR is vital for various cellular processes, including endosomal membrane traffic and calcium-induced endo-lysosome secretion . It also inhibits the Arp2/3 complex function, negatively regulating cell spreading, size, and motility via intracellular sequestration of the Arp2/3 complex . These regulatory functions highlight the importance of AP1AR in maintaining cellular dynamics and ensuring the proper distribution of cellular components .
Research on AP1AR has provided insights into its role in intracellular trafficking and its potential implications in various diseases. For instance, dysregulation of AP1AR function could lead to defects in protein sorting and transport, contributing to the development of certain diseases . Understanding the mechanisms by which AP1AR regulates intracellular trafficking could pave the way for developing therapeutic strategies targeting these pathways.
