EGFR Antibody (PAT2H8AT)
ERBB, ERBB1, Epidermal growth factor receptor, HER1, PIG61, epidermal growth factor receptor, Urogastrone, Proto-oncogene c-ErbB-1, Oncogene ERBB, Cell proliferation inducing protein 61, Cell growth inhibiting protein 40, Avian erythroblastic leukemia viral (verbb) oncogene homolog.
Description
Product Specs
ERBB, ERBB1, Epidermal growth factor receptor, HER1, PIG61, epidermal growth factor receptor, Urogastrone, Proto-oncogene c-ErbB-1, Oncogene ERBB, Cell proliferation inducing protein 61, Cell growth inhibiting protein 40, Avian erythroblastic leukemia viral (verbb) oncogene homolog.
EGFR antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
PAT2H8AT.
Anti-human EGFR mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human EGFR amino acids 424-605 purified from E. coli.
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
The Epidermal Growth Factor Receptor (EGFR) is a transmembrane glycoprotein that plays a crucial role in cellular processes such as proliferation, differentiation, and survival. It is a member of the ErbB family of receptor tyrosine kinases and is activated by binding to its specific ligands, including epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-α).
EGFR consists of three main domains:
- Extracellular Domain: This domain is responsible for ligand binding. It is heavily glycosylated and interacts with EGF and other ligands.
- Transmembrane Domain: This domain anchors the receptor in the cell membrane.
- Intracellular Domain: This domain possesses tyrosine kinase activity, which is essential for signal transduction. Upon ligand binding, the receptor undergoes dimerization and autophosphorylation, leading to the activation of downstream signaling pathways.
Clone PAT2H8AT is a monoclonal antibody specifically designed to target the human EGFR. It is produced by immunizing mice with human EGFR and subsequently fusing the spleen cells of these mice with myeloma cells to create hybridomas. These hybridomas are screened to identify those producing the desired antibody, which is then cloned and expanded.
The mouse anti-human EGFR Clone PAT2H8AT has several applications in research and clinical settings:
- Flow Cytometry: This antibody can be used to detect and quantify EGFR expression on the surface of cells.
- Immunohistochemistry: It is used to visualize EGFR expression in tissue sections, aiding in the diagnosis and study of various cancers.
- Western Blotting: The antibody can be used to detect EGFR in protein extracts from cells and tissues.
- Therapeutic Research: Clone PAT2H8AT is valuable in studying the role of EGFR in cancer and evaluating the efficacy of EGFR-targeted therapies.
EGFR is overexpressed in many types of cancers, including non-small cell lung cancer, colorectal cancer, and head and neck squamous cell carcinoma. Overexpression or mutation of EGFR leads to uncontrolled cell proliferation and survival, contributing to tumor growth and progression. Therefore, targeting EGFR with specific antibodies like Clone PAT2H8AT is a promising strategy for cancer therapy.
