ANKRD54 Human

Ankyrin Repeat Domain 54 Protein Human Recombinant
Cat. No.
BT19331
Source
Escherichia Coli.
Synonyms
LIAR, Lyn-interacting ankyrin repeat protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ANKRD54 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (1-300 a.a.) and having a molecular mass of 34.9kDa.
ANKRD54 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The protein ANKRD54, also referred to as LIAR, possesses 4 ANK repeat domains. It plays a crucial role in the regulation of intracellular signaling pathways associated with the terminal differentiation of erythrocytes. Notably, ANKRD54 interacts with LYN, a protein kinase, through its ankyrin repeat region and LYN's SH3 domain. This interaction occurs in an activation-independent state of LYN. Furthermore, ANKRD54 forms a multiprotein complex by associating with both LYN and HCLS1.
Description
Recombinant human ANKRD54 protein has been produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 323 amino acids (specifically, amino acids 1 to 300). It has a molecular weight of 34.9 kDa. For purification purposes, a 23 amino acid His-tag has been added to the N-terminus of the ANKRD54 protein. The purification process involves proprietary chromatographic techniques.
Physical Appearance
The solution is clear and has been sterilized by filtration.
Formulation
The ANKRD54 protein solution is provided at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For optimal storage, it is recommended to keep the protein solution at 4°C if the entire vial will be used within 2 to 4 weeks. For longer storage periods, the solution should be frozen at -20°C. To ensure long-term stability during storage, adding a carrier protein (either 0.1% HSA or BSA) is advisable. It is crucial to avoid repeated cycles of freezing and thawing to maintain the protein's integrity.
Purity
Analysis by SDS-PAGE indicates a purity level greater than 85.0%.
Synonyms
LIAR, Lyn-interacting ankyrin repeat protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAAAGD ADDEPRSGHS SSEGECAVAP EPLTDAEGLF SFADFGSALG GGGAGLSGRA SGGAQSPLRY LHVLWQQDAE PRDELRCKIP AGRLRRAARP HRRLGPTGKE VHALKRLRDS ANANDVETVQ QLLEDGADPC AADDKGRTAL HFASCNGNDQ IVQLLLDHGA DPNQRDGLGN TPLHLAACTN HVPVITTLLR GGARVDALDR AGRTPLHLAK SKLNILQEGH AQCLEAVRLE VKQIIHMLRE YLERLGQHEQ RERLDDLCTR LQMTSTKEQV DEVTDLLASF TSLSLQMQSM EKR.

Product Science Overview

Function and Role in Cellular Processes

ANKRD54 plays a crucial role in regulating intracellular signaling events, particularly those associated with erythroid terminal differentiation . This protein interacts with LYN, a member of the Src family of tyrosine kinases, through its ankyrin repeat region and the SH3-domain of LYN . This interaction is activation-independent, meaning it occurs regardless of LYN’s activation status . ANKRD54 forms a multiprotein complex with LYN and HCLS1, which is essential for its function in signaling pathways .

Genetic and Disease Associations

The ANKRD54 gene is a protein-coding gene, and its dysfunction has been associated with several diseases. Notably, it is linked to Machado-Joseph Disease and Autosomal Dominant Cerebellar Ataxia . Gene Ontology (GO) annotations related to ANKRD54 include protein-containing complex binding and protein kinase regulator activity . An important paralog of this gene is ANKRD61 .

Structural and Functional Insights

Ankyrin repeats are highly conserved across different species, indicating their functional significance . These repeats are involved in maintaining the integrity of the cytoskeleton and cellular signaling . Ankyrins, including ANKRD54, are modular adaptor proteins that link integral membrane proteins to the spectrin cytoskeleton . This linkage is crucial for various cellular functions, including membrane transport, cellular adhesion, and maintenance of cytoskeleton structures .

Research and Clinical Implications

Research on ANKRD54 and other ankyrin proteins has provided valuable insights into their roles in health and disease. For instance, studies have shown that ankyrins are involved in several diseases, such as hereditary spherocytosis, long QT syndrome, intellectual disability, and CRASH syndrome . Understanding the molecular mechanisms underlying these associations can lead to the development of targeted therapies and improved diagnostic tools.

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