ANGPTL7 Mouse
Description
Product Specs
Product Science Overview
ANGPTL7 is a 45-50 kDa glycoprotein . The recombinant mouse version of this protein is typically produced in Chinese Hamster Ovary (CHO) cell lines and includes a C-terminal 10-His tag for purification purposes . The predicted molecular mass of the recombinant protein is approximately 37.9 kDa, but it often appears as a 45-55 kDa band on SDS-PAGE due to post-translational modifications .
ANGPTL7 has been shown to play a role in various biological processes. One of its key functions is promoting the expansion of E16 rat liver mononuclear cells in vitro . This activity is measured in the presence of other recombinant mouse proteins such as SCF/c-kit Ligand, Thrombopoietin (Tpo), and Flt-3 Ligand . The effective dose (ED50) for this activity ranges from 60-240 ng/mL when cross-linked with a mouse anti-polyHistidine monoclonal antibody .
Recombinant ANGPTL7 is used in various research applications, including cell culture and ELISA standards . It is available in both carrier-free and carrier-containing formulations. The carrier protein, typically Bovine Serum Albumin (BSA), enhances protein stability and shelf-life . However, for applications where the presence of BSA could interfere, the carrier-free version is recommended .
The recombinant protein is lyophilized from a 0.2 μm filtered solution in NaPO4 and NaCl, with or without BSA . It is shipped at ambient temperature and should be stored at -20 to -70 °C upon receipt. After reconstitution, it remains stable for up to 12 months at -20 to -70 °C, and for up to 1 month at 2 to 8 °C under sterile conditions .
